Research Articles

 

 

Tetreau G, Sawaya MR, De Zitter E, Andreeva EA, Banneville AS, Schibrowsky NA, Coquelle N, Brewster AS, Grünbein ML, Kovacs GN, Hunter MS, Kloos M, Sierra RG, Schiro G, Qiao P, Stricker M, Bideshi D, Young ID, Zala N, Engilberge S, Gorel A, Signor L, Teulon JM, Hilpert M, Foucar L, Bielecki J, Bean R, de Wijn R, Sato T, Kirkwood H, Letrun R, Batyuk A, Snigireva I, Fenel D, Schubert R, Canfield EJ, Alba MM, Laporte F, Després L, Bacia M, Roux A, Chapelle C, Riobé F, Maury O, Ling WL, Boutet S, Mancuso A, Gutsche I, Girard E, Barends TRM, Pellequer JL, Park HW, Laganowsky AD, Rodriguez J, Burghammer M, Shoeman RL, Doak RB, Weik M, Sauter NK, Federici B, Cascio D, Schlichting I, Colletier JP. De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals. Nat Commun. 2022 Jul 28;13(1):4376. doi: 10.1038/s41467-022-31746-x.

 

Walker TE, Laganowsky A, Russell DH. Surface Activity of Amines Provides Evidence for the Combined ESI Mechanism of Charge Reduction for Protein Complexes. Anal Chem. 2022 Aug 2;94(30):10824-10831. doi: 10.1021/acs.analchem.2c01814.

 

Zhu Y, Schrecke S, Tang S, Odenkirk MT, Walker T, Stover L, Lyu J, Zhang T, Russell D, Baker ES, Yan X, Laganowsky A. Cupric Ions Selectively Modulate TRAAK-Phosphatidylserine Interactions. J Am Chem Soc. 2022 Apr 27;144(16):7048-7053. doi: 10.1021/jacs.2c00612.

 

Lyu RL, Joy S, Packianathan C, Laganowsky A, Burgess K. Small molecule peptidomimetic trypsin inhibitors: validation of an EKO binding mode, but with a twist. Org Biomol Chem. 2022 Mar 9;20(10):2075-2080.

 

Walker TE, Shirzadeh M, Sun HM, McCabe JW, Roth A, Moghadamchargari Z, Clemmer DE, Laganowsky A, Rye H, Russell DH. Temperature Regulates Stability, Ligand Binding (Mg2+ and ATP), and Stoichiometry of GroEL-GroES Complexes. J Am Chem Soc. 2022 Feb 16;144(6):2667-2678.

 

Abramsson ML, Sahin C, Hopper JTS, Branca RMM, Danielsson J, Xu M, Chandler SA, Österlund N, Ilag LL, Leppert A, Costeira-Paulo J, Lang L, Teilum K, Laganowsky A, Benesch JLP, Oliveberg M, Robinson CV, Marklund EG, Allison TM, Winther JR, Landreh M. Charge Engineering Reveals the Roles of Ionizable Side Chains in Electrospray Ionization Mass Spectrometry. JACS Au. 2021 Nov 29;1(12):2385-2393. doi: 10.1021/jacsau.1c00458

 

Laganowsky A, Clemmer DE, Russell DH. Variable-Temperature Native Mass Spectrometry for Studies of Protein Folding, Stabilities, Assembly, and Molecular Interactions. Annu Rev Biophys. 2021 Dec 21. doi: 10.1146/annurev-biophys-102221-101121

 

Qiao P, Schrecke S, Walker T, McCabe JW, Lyu J, Zhu Y, Zhang T, Kumar S, Clemmer D, Russell DH, Laganowsky A. Entropy in the Molecular Recognition of Membrane Protein-Lipid Interactions. J Phys Chem Lett. 2021 Dec 30;12(51):12218-12224. doi: 10.1021/acs.jpclett.1c03750

 

Qiao P, Schrecke S, Lyu J, Zhu Y, Zhang T, Benavides A, Laganowsky A. Insight into the Phospholipid-Binding Preferences of Kir3.4. Biochemistry. 2021 Dec 21;60(50):3813-3821. doi: 10.1021/acs.biochem.1c00615

 

Implementing Digital-Waveform Technology for Extended m/z Range Operation on a Native Dual-Quadrupole FT-IM-Orbitrap Mass Spectrometer. McCabe JW, Jones BJ, Walker TE, Schrader RL, Huntley AP, Lyu J, Hoffman NM, Anderson GA, Reilly PTA, Laganowsky A, Wysocki VH, Russell DH. J Am Soc Mass Spectrom. 2021 Dec 1;32(12):2812-2820. doi: 10.1021/jasms.1c00245

 

Cong X, Patrick JW, Liu Y, Liang X, Liu W, Laganowsky A. Investigation of Protein-Lipid Interactions Using Native Mass Spectrometry. Methods Mol Biol. 2022;2349:41-64. doi: 10.1007/978-1-0716-1585-0_3

 

Mellott DM, Torres D, Krieger IV, Cameron SA, Moghadamchargari Z, Laganowsky A, Sacchettini JC, Meek TD, Harris LD. Mechanism-Based Inactivation of Mycobacterium tuberculosis Isocitrate Lyase 1 by (2R,3S)-2-Hydroxy-3-(nitromethyl)succinic acid. J Am Chem Soc. 2021 Oct 27;143(42):17666-17676. doi: 10.1021/jacs.1c07970

 

Li L, Chenna BC, Yang KS, Cole TR, Goodall ZT, Giardini M, Moghadamchargari Z, Hernandez EA, Gomez J, Calvet CM, Bernatchez JA, Mellott DM, Zhu J, Rademacher A, Thomas D, Blankenship LR, Drelich A, Laganowsky A, Tseng CK, Liu WR, Wand AJ, Cruz-Reyes J, Siqueira-Neto JL, Meek TD. Self-Masked Aldehyde Inhibitors: A Novel Strategy for Inhibiting Cysteine Proteases. J Med Chem. 2021 Aug 12;64(15):11267-11287. doi: 10.1021/acs.jmedchem.1c00628

 

Raab SA, El-Baba TJ, Laganowsky A, Russell DH, Valentine SJ, Clemmer DE. Protons Are Fast and Smart; Proteins Are Slow and Dumb: On the Relationship of Electrospray Ionization Charge States and Conformations. J Am Soc Mass Spectrom. 2021 Jul 7;32(7):1553-1561. doi: 10.1021/jasms.1c00100

 

El-Baba TJ, Raab SA, Buckley RP, Brown CJ, Lutomski CA, Henderson LW, Woodall DW, Shen J, Trinidad JC, Niu H, Jarrold MF, Russell DH, Laganowsky A, Clemmer DE. Thermal Analysis of a Mixture of Ribosomal Proteins by vT-ESI-MS: Toward a Parallel Approach for Characterizing the Stabilitome. Anal Chem. 2021 Jun 22;93(24):8484-8492. doi: 10.1021/acs.analchem.1c00772

 

McCabe JW, Shirzadeh M, Walker TE, Lin CW, Jones BJ, Wysocki VH, Barondeau DP, Clemmer DE, Laganowsky A, Russell DH. Variable-Temperature Electrospray Ionization for Temperature-Dependent Folding/Refolding Reactions of Proteins and Ligand Binding. Anal Chem. 2021 May 11;93(18):6924-6931. doi: 10.1021/acs.analchem.1c00870

 

Moghadamchargari Z, Shirzadeh M, Liu C, Schrecke S, Packianathan C, Russell DH, Zhao M, Laganowsky A. Molecular assemblies of the catalytic domain of SOS with KRas and oncogenic mutants. Proc Natl Acad Sci U S A. 2021 Mar 23;118(12):e2022403118. doi: 10.1073/pnas.2022403118

 

Pham TV, Mellott DM, Moghadamchargari Z, Chen K, Krieger I, Laganowsky A, Sacchettini JC, Meek TD. Covalent Inactivation of Mycobacterium tuberculosis Isocitrate Lyase by cis-2,3-Epoxy-Succinic Acid. ACS Chem Biol. 2021 Mar 19;16(3):463-470. doi: 10.1021/acschembio.0c00740

 

Schrecke S, Zhu Y, McCabe JW, Bartz M, Packianathan C, Zhao M, Zhou M, Russell D, Laganowsky A. Selective regulation of human TRAAK channels by biologically active phospholipids. Nat Chem Biol. 2021 Jan;17(1):89-95. doi: 10.1038/s41589-020-00659-5

 

Raab SA, El-Baba TJ, Woodall DW, Liu W, Liu Y, Baird Z, Hales DA, Laganowsky A, Russell DH, Clemmer DE. Evidence for Many Unique Solution Structures for Chymotrypsin Inhibitor 2: A Thermodynamic Perspective Derived from vT-ESI-IMS-MS Measurements. JACS. 2020 doi: 10.1021/jacs.0c05365.

 

Khan D, Lee D, Gulten G, Aggarwal A, Wofford J, Krieger I, Tripathi A, Patrick JW, Eckert DM, Laganowsky A, Sacchettini J, Lindahl P, Bankaitis VA. A Sec14-like phosphatidylinositol transfer protein paralog defines a novel class of heme-binding proteins. Elife. 2020;9:e57081. doi: 10.7554/eLife.57081.

 

Lyu J, Liu Y, McCabe JW, Schrecke S, Fang L, Russell DH, Laganowsky A. Discovery of Potent Charge-Reducing Molecules for Native Ion Mobility Mass Spectrometry Studies. Anal Chem. 2020;92(16):11242-11249. doi: 10.1021/acs.analchem.0c01826.

 

McCabe JW, Mallis CS, Kocurek KI, Poltash ML, Shirzadeh M, Hebert MJ, Fan L, Walker TE, Zheng X, Jiang T, Dong S, Lin CW, Laganowsky A, Russell DH. First-Principles Collision Cross Section Measurements of Large Proteins and Protein Complexes. Anal Chem. 2020;92(16):11155-11163. doi: 10.1021/acs.analchem.0c01285.

 

Qiao P, Liu Y, Zhang T, Benavides A, Laganowsky A. Insight into the Selectivity of Kir3.2 toward Phosphatidylinositides. Biochemistry. 2020;59(22):2089-2099. doi: 10.1021/acs.biochem.0c00163.

 

Sipe SN, Patrick JW, Laganowsky A, Brodbelt JS. Enhanced Characterization of Membrane Protein Complexes by Ultraviolet Photodissociation Mass Spectrometry. Anal Chem. 2020 Jan 7;92(1):899-907. doi: 10.1021/acs.analchem.9b03689. Epub 2019 Dec 10.

PMID: 31765130

 

Wang L, Qian H, Nian Y, Han Y, Ren Z, Zhang H, Hu L, Prasad BVV, Laganowsky A, Yan N, Zhou M. Structure and mechanism of human diacylglycerol O-acyltransferase 1. Nature. 2020 May;581(7808):329-332. doi: 10.1038/s41586-020-2280-2. Epub 2020 May 13.

PMID: 32433610

 

Zheng X, Kurulugama RT, Laganowsky A, Russell DH. Collision-Induced Unfolding Studies of Proteins and Protein Complexes using Drift Tube Ion Mobility-Mass Spectrometer. Anal Chem. 2020 May 19;92(10):7218-7225. doi: 10.1021/acs.analchem.0c00772. Epub 2020 May 8. PMID: 32338885

 

Poltash ML, McCabe JW, Shirzadeh M, Laganowsky A, Russell DH. Native IM-Orbitrap MS: Resolving What Was Hidden. Trends Analyt Chem. 2020 Mar;124:115533. doi: 10.1016/j.trac.2019.05.035. Epub 2019 May 31. PMID: 32189816

 

Shirzadeh M, Poltash ML, Laganowsky A, Russell DH. Structural Analysis of the Effect of a Dual-FLAG Tag on Transthyretin. Biochemistry. 2020 Mar 10;59(9):1013-1022. doi: 10.1021/acs.biochem.0c00105. Epub 2020 Mar 2. PMID: 32101399

 

Woodall DW, Brown CJ, Raab SA, El-Baba TJ, Laganowsky A, Russell DH, Clemmer DE. Melting of Hemoglobin in Native Solutions as measured by IMS-MS. Anal Chem. 2020 Feb 18;92(4):3440-3446. doi: 10.1021/acs.analchem.9b05561. Epub 2020 Feb 7.

PMID: 31990187

 

Patrick JW, Laganowsky A. Probing Heterogeneous Lipid Interactions with Membrane Proteins Using Mass Spectrometry. Methods Mol Biol. 2019;2003:175-190. doi: 10.1007/978-1-4939-9512-7_9.

 

Moghadamchargari Z, Huddleston J, Shirzadeh M, Zheng X, Clemmer DE, Raushel F, Russell DH, Laganowsky A, Intrinsic GTPase Activity of K-RAS Monitored by Native Mass Spectrometry. Biochem. 2019 Jul 22. doi: 10.1021/acs.biochem.9b00532. PubMed PMID: 31306575.

 

Woodall D, El-Baba T, Fuller D, Liu W, Brown C, Laganowsky A, Russell D, Clemmer D. Variable-temperature ESI-IMS-MS analysis of myohemerythrin reveals ligand losses, unfolding, and a non-native disulfide bond. Anal Chem. 2019 91(10):6808-6814. doi: 10.1021/acs.analchem.9b00981. PubMed PMID: 31038926.

 

Liu Y, LoCaste CE, Liu W, Poltash ML, Russell DH, Laganowsky A. Selective binding of a toxin and phosphatidylinositides to a mammalian potassium channel. Nat Commun. 2019 Mar 22;10(1):1352. doi: 10.1038/s41467-019-09333-4. PubMed PMID: 30902995.

 

Poltash ML, Shirzadeh M, McCabe JW, Moghadamchargari Z, Laganowsky A, Russell DH. New insights into the metal-induced oxidative degradation pathways of transthyretin. Chem Commun (Camb). 2019 Apr 2;55(28):4091-4094. doi:

10.1039/c9cc00682f. PubMed PMID: 30887985.

 

Patrick JW, Laganowsky A. Generation of Charge-Reduced Ions of Membrane Protein Complexes for Native Ion Mobility Mass Spectrometry Studies. J Am Soc Mass Spectrom. 2019 Mar 18. doi: 10.1007/s13361-019-02187-6. PubMed PMID: 30887461.

 

Zhao B, Du F, Shu C, Sankaran B, Bell SL, Liu M, Lei Y, Gao X, Fu X, Fanxiu Z, Liu Y, Laganowsky, A, Zheng X, Ji J, West AP, Watson RO, Li P. A Conserved PLPLRT/SD Motif of STING Mediates the Recruitment and Activation of TBK1. Nature. 2019 569(7758):718-722. doi: 10.1038/s41586-019-1228-x. PubMed PMID: 31118511

 

Shirzadeh M, Boone CD, Laganowsky A, Russell DH. Topological Analysis of Transthyretin Disassembly Mechanism: Surface-Induced Dissociation Reveals Hidden Reaction Pathways. Anal Chem. 2019 Feb 5;91(3):2345-2351. doi: 10.1021/acs.analchem.8b05066. Epub 2019 Jan 28. PubMed PMID: 30642177.

 

Poltash ML, McCabe JW, Shirzadeh M, Laganowsky A, Clowers BH, Russell DH. Fourier Transform-Ion Mobility-Orbitrap Mass Spectrometer: A Next-Generation Instrument for Native Mass Spectrometry. Anal Chem. 2018 Sep 4;90(17):10472-10478. doi: 10.1021acs.analchem.8b02463. Epub 2018 Aug 22. PubMed PMID: 30091588.

 

Poltash ML, McCabe JW, Patrick JW, Laganowsky A, Russell DH. Development and Evaluation of a Reverse-Entry Ion Source Orbitrap Mass Spectrometer. J Am Soc Mass Spectrom. 2018 May 23. doi: 10.1007/s13361-018-1976-0. [Epub ahead of print] PubMed PMID: 29796735.

 

Patrick JW, Boone CD, Liu W, Conover GM, Liu Y, Cong X, Laganowsky A. Allostery revealed within lipid binding events to membrane proteins. Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):2976-2981. doi: 10.1073/pnas.1719813115. Epub 2018 Mar 5. PubMed PMID: 29507234.

 

Hochberg GKA, Shepherd DA, Marklund EG, Santhanagoplan I, Degiacomi MT, Laganowsky A, Allison TM, Basha E, Marty MT, Galpin MR, Struwe WB, Baldwin AJ, Vierling E, Benesch JLP. Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions. Science. 2018 Feb 23;359(6378):930-935. doi: 10.1126/science.aam7229. PubMed PMID: 29472485.

 

Bergdoll LA, Lerch MT, Patrick JW, Belardo K, Altenbach C, Bisignano P, Laganowsky A, Grabe M, Hubbell WL, Abramson J. Protonation state of glutamate 73 regulates the formation of a specific dimeric association of mVDAC1. Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E172-E179. doi: 10.1073/pnas.1715464115. Epub 2017 Dec 26. PubMed PMID: 29279396

 

Cong X, Liu Y, Liu W, Liang X, Laganowsky A. Allosteric modulation of protein-protein interactions by individual lipid binding events. Nat Commun. 2017 Dec 19;8(1):2203. doi: 10.1038/s41467-017-02397-0. PubMed PMID: 29259178

 

El-Baba TJ, Woodall DW, Raab SA, Fuller DR, Laganowsky A, Russell DH, Clemmer  DE. Melting Proteins: Evidence for Multiple Stable Structures upon Thermal Denaturation of Native Ubiquitin from Ion Mobility Spectrometry-Mass Spectrometry Measurements. J Am Chem Soc. 2017 May 10;139(18):6306-6309. doi: 10.1021/jacs.7b02774. Epub 2017 Apr 26. PubMed PMID: 28427262.

 

Harvey SR, Liu Y, Liu W, Wysocki VH, Laganowsky A. Surface induced dissociation as a tool to study membrane protein complexes. Chem Commun (Camb). 2017 Mar 9;53(21):3106-3109. doi: 10.1039/c6cc09606a. PubMed PMID: 28243658.

 

Liu Y, Cong X, Liu W, Laganowsky A. Characterization of Membrane Protein-Lipid Interactions by Mass Spectrometry Ion Mobility Mass Spectrometry. J Am Soc Mass Spectrom. 2016 Dec 6. [Epub ahead of print] PubMed PMID: 27924494.

 

Cong X, Liu Y, Liu W, Liang X, Russell DH, Laganowsky A. Determining Membrane Protein-Lipid Binding Thermodynamics Using Native Mass Spectrometry. J Am Chem Soc. 2016 Apr 6;138(13):4346-9. doi: 10.1021/jacs.6b01771. Epub 2016 Mar 25. PubMed PMID: 27015007.

 

Allison TM, Reading E, Liko I, Baldwin AJ, Laganowsky A, Robinson CV. Quantifying the stabilizing effects of protein-ligand interactions in the gas phase. Nat Commun. 2015 Oct 6;6:8551. doi: 10.1038/ncomms9551. PubMed PMID: 26440106; PubMed Central PMCID: PMC4600733.

 

Reading E, Walton TA, Liko I, Marty MT, Laganowsky A, Rees DC, Robinson CV. The Effect of Detergent, Temperature, and Lipid on the Oligomeric State of MscL Constructs: Insights from Mass Spectrometry. Chem Biol. 2015 May 21;22(5):593-603. doi:10.1016/j.chembiol.2015.04.016. PubMed PMID: 26000747.

 

Reading E, Liko I, Allison TM, Benesch JL, Laganowsky A, Robinson CV. The role of the detergent micelle in preserving the structure of membrane proteins in the gas phase. Angew Chem Int Ed Engl. 2015 Apr 7;54(15):4577-81. doi: 10.1002/anie.201411622. Epub 2015 Feb 18. PubMed PMID: 25693501.

 

Lai YT, Reading E, Hura GL, Tsai KL, Laganowsky A, Asturias FJ, Tainer JA, Robinson CV, Yeates TO. Structure of a designed protein cage that self-assembles into a highly porous cube. Nat Chem. 2014 Dec;6(12):1065-71. doi: 10.1038/nchem.2107. Epub 2014 Nov 10. PubMed PMID: 25411884; PubMed Central PMCID: PMC4239666.

 

Laganowsky A,Ф, § Reading E, Allison TM, Ulmschneider MB, Degiacomi MT, Baldwin AJ, Robinson CV. Membrane proteins bind lipids selectively to modulate their structure and function. Nature. 2014 Jun 5;510(7503):172-5. doi: 10.1038/nature13419. PubMed PMID: 24899312; PubMed Central PMCID: PMC4087533. ФEqual authorship, §Corresponding author. (Cover Article)

 

Hochberg GK, Ecroyd H, Liu C, Cox D, Cascio D, Sawaya MR, Collier MP, Stroud J, Carver JA, Baldwin AJ, Robinson CV, Eisenberg DS, Benesch JL, Laganowsky A. § The structured core domain of αB-crystallin can prevent amyloid fibrillation and associated toxicity. Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):E1562-70. doi: 10.1073/pnas.1322673111. Epub 2014 Apr 7. PubMed PMID: 24711386; PubMed Central PMCID: PMC4000792. §Corresponding author.

 

Hopper JT, Yu YT, Li D, Raymond A, Bostock M, Liko I, Mikhailov V, Laganowsky A, Benesch JL, Caffrey M, Nietlispach D, Robinson CV. Detergent-free mass spectrometry of membrane protein complexes. Nat Methods. 2013 Dec;10(12):1206-8. doi: 10.1038/nmeth.2691. Epub 2013 Oct 13. PubMed PMID: 24122040; PubMed Central PMCID: PMC3868940. (Cover Article)

 

Hilton GR, Hochberg GK, Laganowsky A, McGinnigle SI, Baldwin AJ, Benesch JL. C-terminal interactions mediate the quaternary dynamics of αB-crystallin. Philos Trans R Soc Lond B Biol Sci. 2013 Mar 25;368(1617):20110405. doi: 10.1098/rstb.2011.0405. Print 2013 May 5. PubMed PMID: 23530258; PubMed Central PMCID: PMC3638394.

 

Laganowsky A, Reading E, Hopper JT, Robinson CV. Mass spectrometry of intact membrane protein complexes. Nat Protoc. 2013 Apr;8(4):639-51. doi: 10.1038/nprot.2013.024. Epub 2013 Mar 7. PubMed PMID: 23471109; PubMed Central PMCID: PMC4058633.

 

Gallat FX, Laganowsky A, Wood K, Gabel F, van Eijck L, Wuttke J, Moulin M, Härtlein M, Eisenberg D, Colletier JP, Zaccai G, Weik M. Dynamical coupling of intrinsically disordered proteins and their hydration water: comparison with folded soluble and membrane proteins. Biophys J. 2012 Jul 3;103(1):129-36. doi: 10.1016/j.bpj.2012.05.027. PubMed PMID: 22828339; PubMed Central PMCID: PMC3388209.

 

Senturia R, Laganowsky A, Barr I, Scheidemantle BD, Guo F. Dimerization and heme binding are conserved in amphibian and starfish homologues of the microRNA processing protein DGCR8. PLoS One. 2012;7(7):e39688. doi: 10.1371/journal.pone.0039688. Epub 2012 Jul 2. PubMed PMID: 22768307; PubMed Central PMCID: PMC3388087.

 

Laganowsky A, Liu C, Sawaya MR, Whitelegge JP, Park J, Zhao M, Pensalfini A, Soriaga AB, Landau M, Teng PK, Cascio D, Glabe C, Eisenberg D. Atomic view of a toxic amyloid small oligomer. Science. 2012 Mar 9;335(6073):1228-31. doi: 10.1126/science.1213151. PubMed PMID: 22403391; PubMed Central PMCID: PMC3959867.

• Covered by Science in a ‘perspective’ article “Visualizing Amyloid Assembly”, Science 2012, 336:308-309
• Covered by Nature Structural & Molecular Biology in Research Highlights “A glimpse at toxicity ”NSMB 2012, 19:369
• Covered by Science in ‘This week in Science’ article “A Toxic Barrel”, Science 2012, 335:1146
• Covered at the Alzheimer Research Forum (http://www.alzforum.org/new/detail.asp?id=3094)
• Ranked “must read” in Faculty of 1000

 

Colletier JP, § Laganowsky A, § Landau M, § Zhao M, Soriaga AB, Goldschmidt L, Flot D, Cascio D, Sawaya MR, Eisenberg D. Molecular basis for amyloid-beta polymorphism. Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):16938-43. doi: 10.1073/pnas.1112600108. Epub 2011 Sep 23. PubMed PMID: 21949245; PubMed Central PMCID: PMC3193189. §Equal authorship

 

Laganowsky A, Zhao M, Soriaga AB, Sawaya MR, Cascio D, Yeates TO. An approach to crystallizing proteins by metal-mediated synthetic symmetrization. Protein Sci. 2011 Nov;20(11):1876-90. doi: 10.1002/pro.727. Epub 2011 Sep 30. PubMed PMID: 21898649; PubMed Central PMCID: PMC3267952. Ranked “must read” in Faculty of 1000.

 

Landau M, Sawaya MR, Faull KF, Laganowsky A, Jiang L, Sievers SA, Liu J, Barrio JR, Eisenberg D. Towards a pharmacophore for amyloid. PLoS Biol. 2011 Jun;9(6):e1001080. doi: 10.1371/journal.pbio.1001080. Epub 2011 Jun 14. PubMed PMID: 21695112; PubMed Central PMCID: PMC3114762.

 

Webb KJ, Zurita-Lopez CI, Al-Hadid Q, Laganowsky A, Young BD, Lipson RS, Souda P, Faull KF, Whitelegge JP, Clarke SG. A novel 3-methylhistidine modification of yeast ribosomal protein Rpl3 is dependent upon the YIL110W methyltransferase. J Biol Chem. 2010 Nov 26;285(48):37598-606. doi: 10.1074/jbc.M110.170787. Epub 2010 Sep 23. PubMed PMID: 20864530; PubMed Central PMCID: PMC2988365.

 

Laganowsky A, Eisenberg D. Non-3D domain swapped crystal structure of truncated zebrafish alphaA crystallin. Protein Sci. 2010 Oct;19(10):1978-84. doi: 10.1002/pro.471. PubMed PMID: 20669149; PubMed Central PMCID: PMC2998731.

 

Laganowsky A, Benesch JL, Landau M, Ding L, Sawaya MR, Cascio D, Huang Q, Robinson CV, Horwitz J, Eisenberg D. Crystal structures of truncated alphaA and alphaB crystallins reveal structural mechanisms of polydispersity important for eye lens function. Protein Sci. 2010 May;19(5):1031-43. vdoi: 10.1002/pro.380. PubMed PMID: 20440841; PubMed Central PMCID: PMC2868245.

 

Puppione DL, Donna LD, Laganowsky AD, Bassilian S, Souda P, Ryder OA, Whitelegge JP. Mass spectral analyses of the two major apolipoproteins of great ape high density lipoproteins. Comp Biochem Physiol Part D Genomics Proteomics. 2009 Dec;4(4):305-9. PubMed PMID: 21298813; PubMed Central PMCID: PMC2776726.

 

Laganowsky A, Gómez SM, Whitelegge JP, Nishio JN. Hydroponics on a chip: analysis of the Fe deficient Arabidopsis thylakoid membrane proteome. J Proteomics. 2009 Apr 13;72(3):397-415. PubMed PMID: 19367733.

 

Webb KJ, Laganowsky A, Whitelegge JP, Clarke SG. Identification of two SET domain proteins required for methylation of lysine residues in yeast ribosomal protein Rpl42ab. J Biol Chem. 2008 Dec 19;283(51):35561-8. doi: 10.1074/jbc.M806006200. Epub 2008 Oct 28. PubMed PMID: 18957409; PubMed Central PMCID: PMC2602916.

 

Whitelegge JP, Laganowsky A, Nishio J, Souda P, Zhang H, Cramer WA. Sequencing covalent modifications of membrane proteins. J Exp Bot. 2006;57(7):1515-22. Epub 2006 Mar 30. PubMed PMID: 16574746.

 

Book Chapters

 

Faull KF, Dooley AN, Halgand F, Shoemaker LD, Norris AJ, Ryan CM, Laganowsky A, Johnson JV, Katz JE. “An Introduction to the basic principles and concepts of mass spectrometry.” In: D. Barcelo, editor: Comprehensive Analytical Chemistry, Vol 52, Protein Mass Spectrometry, Julian P. Whitelegge. Elsevier BV: Elsevier, 2009, pp. 1–46. ISBN: 9780444530554