Quantitative Analysis Laboratory (0-3) Credit 1.  I, II, SSI

Determinations are designed to illustrate the theory and practice of volumetric and instrumental methods of quantitative analyses.  In all analyses, the students are expected to statistically examine their results with respect to the precision obtained.  It is anticipated that students will learn the care necessary at every step of an analysis in order to obtain accurate results.  Thus grades are based in large part on accuracy of the student measurement.

Laboratory Coordinator: Marvin W. Rowe, room 101A, Analytical Services Bldg., 845-1929; mwrowe@tamu.edu

Text:  Chemistry 318 Quantitative Analysis Laboratory Manual, 5th edition, Burgess Publishing, M. W. Rowe, M. Hyman and Ann E. Miller, 1999.

Experiments done:
1. Acid-Base Titrimetry - Analysis of KHP by titration with standardized NaOH.
2. Determination of Iron - Titration of Fe(NH3)2SO4 with a standard K2Cr2O7 solution .
3. Determination of Chloride-Fajans Method - Titration of Cl- with standard AgNO3 solution.
4. Analysis of Vitamin C - Ascorbic acid content is determined by titration of excess I2, produced in situ by a known amount of KIO3 and excess NaI, with a standard Na2S2O3 solution.
5. Determination of Total Salt by Ion Exchange - Dowex 50 cation exchange resin is used in a conventional column to produce H+ equivalent to the quantity of cation exchanged.  The acid produced is titrated with standardized NaOH.
6. Kjeldahl Ammonium Analysis - Reagent grade NH4+ salts are analyzed.  The NH3 produced is reacted with H3BO3 and the product titrated with standard HCl.
7. Analysis of Magnesium by EDTA - Mg is analyzed by titration with standard EDTA.
8. Conductometric Titration - Na2CO3 is titrated with standard HCl solution and conduc-tance measurements taken during the course of the titration with a conventional conductance bridge.  The end point is determined graphically.
9. Determination of Serum Glucose - Standard glucose solutions are prepared, a color developed with o-toluidine, absorbance measurements taken using a Spectronic 20 and a Beer’s Law plot obtained.  The glucose content of a prepared serum sample is obtained by comparison.
10. Spectrophotometric Studies of the Iron (III) – Sulfosalicylic Acid- Complex Ion - The method of continuous variations is used to determine the formula of the complex.  Values of the molar extinction coefficient are calculated and an average determined.  The stability constant, Kf, is calculated from the measured and theoretical values at the stoichiometric point.  The percent iron in an unknown sample treated with excess ligand is calculated by use of Beer’s Law.
11. Potentiometric Titration - Na2CO3 is titrated with standardized HCl.  pH values are measured during the titration with the end point being determined graphically.
12. Determination of Fluoride - The potentials of standard solutions of NaF are measured using a F- specific electrode, a Ag/AgCl reference electrode and a pH meter functioning as a potentiometer.  F- in water is similarly treated and its concentration determined graphically.

Prerequisites: Chemistry 102 or 114; Chemistry 316 or 315 or registration therein.  With con-current registration, a student cannot drop Chemistry 316 or 315 and continue Chemistry 318.

Grading Scheme:
(a) 3 points for attending the laboratory period, collecting the data and turning in the data sheets to the TA in charge of the laboratory
(b) 1 point for leaving a clean laboratory space
(c) 1 point for using the correct format for the turned in report
(d) 5 points for the closeness of student’s results with the correct value
The final grade will be the average of the laboratory reports after dropping the lowest two non-zero grades, where A = 9.0 - 10;  B = 8.0 - 8.9;  C = 7.0 - 7.9;  D = 6.0 - 6.9;  F = 0 - 5.9.  Only excused absences will be allowed zero drops.

No grade <4 will be dropped except for excused reasons; for excused absences, you must contact Prof. Rowe within two working days after you are allowed to return to school (Section 7.5, Student Rules, 1998-1999.  It does not suffice to talk to your TA; you must contact Prof. Rowe.  There will be no make-up laboratories.  The teaching assistant assigned to your laboratory section will be there to assist you in any way that he/she can, whether in the mechanics of the experiment or in the calculations.
If you wish to discuss any problems with regard to this laboratory, please feel free to contact me at the Old State Chemistry Building, room 101A or, by telephone at 845-1929, or by e-mail at mwrowe@tamu.edu.

Assignments: It is expected that students will have studied an experiment to be done in laboratory in advance of attending the laboratory.

Handouts used in this course are copyrighted.  By "handouts," I mean all materials generated for this class, which include but are not limited to syllabi, quizzes, exams, lab problems, in-class materials, review sheets, and additional problem sets.  Because these materials are copyrighted, you do not have the right to copy the handouts, unless I expressly grant permission.

As commonly defined, plagiarism consists of passing off as one’s own the idea, words, writings, etc., that belong to another.  In accordance with this definition, you are committing plagiarism if you copy the work of another person and turn it in as you own, even if you should have the permission of that person.  Plagiarism is one of the worst academic sins, for the plagiarist destroys the trust among colleagues without which research cannot be safely communicated.

If you have any questions regarding plagiarism, please consult the latest issue of the Texas A&M University Student Rules, under the section "Scholastic Dishonesty."

The Americans with Disabilities Act (ADA) is a federal anti-discrimination stature that provides comprehensive civil rights protection for persons with disabilities.  Among other things, this legis-lation requires that all students with disabilities be guaranteed a learning environment that provides for a reasonable accommodation of their disabilities.  If you believe you have a disability requiring an accommodation, please contact the Department of Student Life, Services for Students with Disabilities in Room 126 of the Koldus Building.  The phone number is 845-1637.