Department of Chemistry

Responsive image

Frank M. Raushel

Publications 1991 to 1995


104.  T. O. Baldwin, J. A. Christopher, F. M. Raushel, J. F. Sinclair, M. M. Zeigler, A. J. Fisher, and I. Rayment, “Structure of Bacterial LuciferaseCurrent Opinion in Structural Biology 5, 798-809 (1995).


103.  L. S. Mullins, M. Y. Chae, and F. M. Raushel, “Phosphorus-31 NMR Relaxation Studies of Diethyl p-Methoxyphenyl Phosphate Bound to PhosphotriesteraseBioorganic and Medicinal Chemistry Letters 5, 3067-3072 (1995).


102.  R. M. Czerwinski, S. M. Mareya, and F. M. Raushel, “Regulatory Changes in the Control of Carbamoyl Phosphate Synthetase Induced by Truncation and Mutagenesis of the Allosteric Binding DomainBiochemistry 34, 13920-13927 (1995).


101.  S.-B. Hong, J. M. Kuo, L. S. Mullins, F. M. Raushel, “CO2 is Required for the Assembly of the Binuclear Metal Center of PhosphotriesteraseJournal of American Chemical Society 117, 7581-7582 (1995).


100.  M. M. Benning, J. M. Kuo, F. M. Raushel, H. M. Holden, “Three-Dimensional Structure of the Binuclear Metal Center of PhosphotriesteraseBiochemistry 34, 7973-7978 (1995).


99.  A. J. Fisher, F. M. Raushel, T. O. Baldwin, and I. Rayment, “The Three-dimensional Structure of Bacterial Luciferase from Vibrio harveyi at 2.4 Å ResolutionBiochemistry 34, 6581-6586 (1995).


98.  F. M. Raushel, “Structural and Mechanistic Investigations of Carbamoyl Phosphate Synthetase from E. coliPathways to Pyrimidines: An International Newsletter 3, 1-8 (1995).

 

97.  S. M. Mareya and F. M. Raushel, “Mapping the Functional Domains of E. coli Carbamoyl Phosphate Synthetase Using Limited ProteolysisBioorganic and Medicinal Chemistry 3, 525-532 (1995).


96.  J. M. Kuo, L. S. Mullins, J. B. Garrett, and F. M. Raushel, “Circular Permutation of RNase T1 Through PCR Based Site-Directed MutagenesisTechniques in Protein Chemistry VI  (J. W. Crabb, ed) Academic Press  pp. 333-340 (1995).


95.  J. A. Banzon, J. M. Kuo, D. R. Fischer, P. J. Stang and F. M. Raushel, “Histidine-254 of Phosphotriesterase is Essential for Inactivation with the Alkynyl Phosphate Esters and Diethyl Pyrocarbonate" Biochemistry 34, 750-754 (1995).


94.  J. A. Banzon, J. M. Kuo, B. W. Miles, D. R. Fischer, P. J. Stang, and F. M. Raushel, “Mechanism-Based Inactivation of Phosphotriesterase by Reaction of a Critical Histidine with a Ketene Intermediate" Biochemistry 34, 743-749 (1995).

 

93.  M. Y. Chae, G. A. Omburo, P. A. Lindahl, and F. M. Raushel, "Utilization of Copper as a Paramagnetic Probe for the Binuclear Metal Center of Phosphotriesterase" Archives Biochemistry & Biophysics 316, 765-772 (1995).


92.  J. Thoden, F. M. Raushel, S. Mareya, D. R. Tomchick, and I. Rayment, "Crystallization and Preliminary X-ray Crystallographic Analysis of Carbamoyl Phosphate Synthetase from Escherichia coli"  Acta Crystallographica, Part D 51, 827-829 (1995).

 

91.  L. S. Mullins and F. M. Raushel, "Positional Isotope Exchange as a Probe of Enzyme Action"  in Enzyme Kinetics and Mechanism, Part D (Ed. D. L. Purich) Academic Press 249, 398-425 Methods in Enzymology (1995).


90.  S.-B Hong and F. M. Raushel, “Synthesis and Enzymatic Hydrolysis of a Light-Emitting Substrate for PhosphotriesteraseBioorganic and Medicinal Chemistry Letters 4, 2705-2708 (1994).


89.    M. A. Benning, J. M. Kuo, F. M. Raushel, H. M. Holden, “The Three-Dimensional Structure of Phosphotriesterase:  An Enzyme Capable of Detoxifying Organophosphorus Nerve Agents" Biochemistry 33, 15001-15007 (1994).


88.  W. A. Francisco, H. M. Abu-Soud, F. M. Raushel, T. O. Baldwin, "Studies on the Kinetic Mechanism of the Bacterial Luciferase-Catalyzed Reaction" in Bioluminescence and Chemiluminescence (A. A. Szalay, L. J. Kricka, and P. Stanley, eds) pp. 113-117 (1994)

 

87.  M. Y. Chae, J. F. Postula, and F. M. Raushel, "Stereospecific Enzymatic Hydrolysis of Phosphorus-Sulfur Bonds in Chiral Organophosphate Triesters," Bioorganic and Medicinal Chemistry Letters 4, 1473-1478 (1994).

 

86.  L. Pei, G. Omburo, W. D. McGuinn, I. Perikovics, K. Dave, F. M. Raushel, J. R. Wild, J. R. DeLoach, and J. L. Way, "Encapsulation of Phosphotriesterase Within Murine Erythrocytes",  Toxicology and Applied Pharmacology 124, 296-301 (1994).


85.  L. S. Mullins, K. Wesseling, J. M. Kuo, J. B. Garrett, and F. M. Raushel, "Transposition of Protein Sequences: Circular Permutation of Ribonuclease T1" Journal of American Chemical Society 116, 5529-5233 (1994).

 

84.  J. M. Kuo and F. M. Raushel, "Identification of the Histidine Ligands to the Binuclear Metal Center of Phosphotriesterase by Site-Directed Mutagenesis" Biochemistry 33, 4265-4272 (1994).


83.   S. M. Mareya and F. M. Raushel, "A Molecular Wedge for Triggering the Amidotransferase Activity of Carbamoyl Phosphate Synthetase" Biochemistry 33, 2945-2950 (1994).

 

82.  W. A. Francisco, H. M. Abu-Soud, A. C. Clark, F. M. Raushel, and T. O.Baldwin , "Kinetic Mechanism of the Bacterial Luciferase Reaction" in Flavins and Flavoproteins, 1993 (K. Yagi, ed) Walter Gruyter & Co. Berlin pp.829-837 (1994).


81.  K. Tuovinen, E. Kaliste-Korhonen, F. M. Raushel, and O. Hanninen, "Phosphotriesterase - A Promising Candidate for use in Detoxification of Organophosphates" Fundamental and Applied Toxicology 23, 578-584 (1994).


80.  M. Y. Chae, G. E. Omburo, P. A. Lindahl, and F. M. Raushel, "Antiferromagnetic Coupling in the Binuclear Metal Center of Mn-Substituted Phosphotriesterase" Journal of  American Chemical Society 115, 12173-12174 (1993).

 

79.  W. A. Francisco, H. Abu-Soud, T. O. Baldwin and F. M. Raushel, "Interaction of Aldehyde Substrates and Inhibitors to Bacterial Luciferase" Journal of Biological Chemistry 268, 24734-24741 (1993).


78.  G. M. Omburo, L. S. Mullins and F. M. Raushel, "Structural Characterization of the Divalent Cation Sites of Bacterial Phosphotriesterase by 113Cd NMR Spectroscopy" Biochemistry 32, 9148-9155 (1993).

 

77.  L. S. Mullins, C. N. Pace, and F. M. Raushel, "Investigation of Ribonuclease T1 Folding by Hydrogen Deuterium Amide Exchange-2D NMR Spectroscopy" Biochemistry 32, 6152-6156 (1993).

 

76.  R. Rajapaksa, H. Abu-Soud, F. M. Raushel, B. G. Harris, and P. F. Cook, "Pre-steady State Kinetics Reveal a Slow Isomerization of the Enzyme-NAD Complex in the NAD-Malic Enzyme Reaction" Biochemistry 32, 1928-1934 (1993).

 

75.  E. J. Rodriguez, C. Debouck, I. Deckman, H. Abu-Soud, F. M. Raushel, and T. D. Meek, "Inhibitor Binding to the Phe53Trp Mutant of HIV-1 Protease Promotes Conformational Changes Detectable by Spectrofluorimetry" Biochemistry 32, 3557-3563 (1993).

 

74. H. M. Abu-Soud, A. C. Clark, W. A. Francisco, T. O. Baldwin, and F. M. Raushel, "Kinetic Destabilization of the Hydroperoxy Flavin Intermediate by Site-Directed Modification of the Reactive Thiol in Bacterial Luciferase" Journal of Biological Chemistry 268, 7699-7706 (1993).


73.  E. Kaliste-Korhonen, P. Ylitalo, O. Hanninen, and F. M. Raushel "Phosphotriesterase Decreases Paraoxon Toxicity in Mice" Toxicology & Applied Pharmacology 121, 275-278 (1993).

 

72.  B. W. Miles, S. M. Mareya, L. E. Post, D. J. Post, S. H. Chang, and F. M. Raushel, "Differential Roles for Three Conserved Histidine Residues Within the Large Subunit of Carbamoyl Phosphate Synthetase" Biochemistry 32, 232-240 (1993).

 

71. F. M. Raushel, B. W. Miles, L. E. Post and D. J. Post, "Mutational Analysis of Two Putative Domains Within the Large Subunit of Carbamoyl Phosphate Synthetase from E. coli" Bioorganic & Medicinal Chemistry Letters 2, 319-322 (1992).


70.  G. A. Omburo, J. M. Kuo, L. S. Mullins, and F. M. Raushel, "Characterization of the Zinc Binding Site of Bacterial Phosphotriesterase" Journal of Biological Chemistry 267, 13278-13283 (1992).


69.  B. L. Braxton, L. S. Mullins, F. M. Raushel, G. D. Reinhart, "Quantifying the Allosteric Properties of E. coli Carbamoyl Phosphate Synthetase" Biochemistry 31, 2309-2316 (1992).


68.  H. M. Abu-Soud, L. S. Mullins, T. O. Baldwin, and F. M. Raushel, "A Stopped-Flow Kinetic Analysis of the Bacterial Luciferase Reaction" Biochemistry 31, 3807-3813 (1992).


67.  T. O. Baldwin, F. M. Raushel, and A. I. Scott, eds. "Chemical Aspects of Enzyme Biotechnology: Fundamentals" Plenum Press, New York, 359 pp. (1991).


66.  J. N. Blankenship, H. M. Abu-Soud, W. A. Francisco, F. M. Raushel, D. R. Fischer, and P. J. Stang, "Mechanism-Based Inactivation of a Bacterial Phosphotriesterase by an Alkynyl Phosphate Ester" Journal of American Chemical Society 113, 8560-8561 (1991).


65.  S. G. Miran, S. H. Chang, and F. M. Raushel, "The Role of the Four Conserved Histidine Residues in the Amidotransferase Domain of Carbamoyl Phosphate Synthetase" Biochemistry 30, 7901-7907 (1991).


64.  S. R. Caldwell and F. M. Raushel "Detoxification of Organophosphate Pesticides Using a Nylon Based Immobilized Phosphotriesterase From Pseudomonas Diminuta" Applied Biochemistry & Biotechnology 31, 59-73 (1991).

 

63.  S. R. Caldwell, F. M. Raushel, P. M. Weiss, and W. W. Cleland, "Transition State Structures for the Enzymatic and Chemical Hydrolysis of Phosphotriesters" Biochemistry 30, 7444-7450 (1991).


62. S. R. Caldwell, J. R. Newcomb, K. A. Schlecht, and F. M. Raushel, "Limits of Diffusion in the Hydrolysis of Substrates by the Phosphotriesterase from Pseudomonas diminuta" Biochemistry 30, 7438-7444 (1991).

 

61. S. R. Caldwell, F. M. Raushel, P. M. Weiss, and W. W. Cleland, "Primary and Secondary Oxygen-18 Isotope Effects in the Chemical and Enzymatic Hydrolysis of Phosphotriesters" Journal of American Chemical Society 113, 730-732 (1991).


60. L. J. Chlumsky, L. H. Chen, C. Clark, H. Abu-Soud, M. M. Zeigler, F. M. Raushel, and T. O. Baldwin, "Random and Site Directed Mutagenesis of Bacterial Luciferase" in Flavins and Flavoproteins 1990 (Curti, Ronchi, and Zanetti, Eds.) Walter De Gruter & Co. 261-264 (1991).


59.  F. M. Raushel, H. M. Abu-Soud, L. S. Hester, W. Francisco and T. O. Baldwin, "Kinetic Mechanism of Bacterial Luciferase" in Flavins and Flavoproteins 1990 (Curti, Ronchi, and Zanetti, Eds.) Walter De Gruter & Co. 273-276 (1991).


 58.  S. R. Caldwell and F. M. Raushel, "Mechanism of Enzymatic Phosphotriester Hydrolysis", in Chemical Aspects of Enzyme Biotechnology: Fundamentals (T. O. Baldwin, F. M. Raushel, and A. I. Scott eds.) Plenum Press 41-52 (1991).


57.  S. R. Caldwell and F. M. Raushel, "Detoxification of Organophosphate Pesticides Using an Immobilized Phosphotriesterase from Pseudomonas diminuta" Biotechnology and Bioengineering 37, 103-109 (1991).


56.  L. S. Mullins, C. J. Lusty and F. M. Raushel, "Alterations in the Energetics of Carbamoyl Phosphate Synthetase Reaction by Site-Directed Modification of the Essential Sulfhydryl Group" Journal of Biological Chemistry 266, 8236 (1991).