List of Publications
2013
Bigley, AN, Xu, C, Henderson, TJ, Harvey, SP, Raushel, FM (2013) Enzymatic Neutralization of the Chemical Warfare Agent VX: Evolution of Phosphotriesterase for Phosphorothiolate Hydrolysis. J. Am. Chem. Soc. :
Kamat, SS, Williams, HJ, Dangott, LJ, Chakrabarti, M, Raushel, FM (2013) The catalytic mechanism for aerobic formation of methane by bacteria. Nature 497: 132-6
Ghodge, SV, Fedorov, AA, Fedorov, EV, Hillerich, B, Seidel, R, Almo, SC, Raushel, FM (2013) Structural and Mechanistic Characterization of l-Histidinol Phosphate Phosphatase from the Polymerase and Histidinol Phosphatase Family of Proteins. Biochemistry 52: 1101-12Fan, H, Hitchcock, DS, Seidel, RD 2nd, Hillerich, B, Lin, H, Almo, SC, Sali, A, Shoichet, BK, Raushel, FM (2013) Assignment of pterin deaminase activity to an enzyme of unknown function guided by homology modeling and docking. J. Am. Chem. Soc. 135: 795-803
Hobbs, ME, Vetting, M, Williams, HJ, Narindoshvili, T, Kebodeaux, DM, Hillerich, B, Seidel, RD, Almo, SC, Raushel, FM (2013) Discovery of an L-fucono-1,5-lactonase from cog3618 of the amidohydrolase superfamily. Biochemistry 52: 239-53
Ornelas, A, Korczynska, M, Ragumani, S, Kumaran, D, Narindoshvili, T, Shoichet, BK, Swaminathan, S, Raushel, FM (2013) Functional annotation and three-dimensional structure of an incorrectly annotated dihydroorotase from cog3964 in the amidohydrolase superfamily. Biochemistry 52: 228-38
2012
Tsai PC, Fox N, Bigley AN, Harvey SP, Barondeau DP & Raushel FM (2012) Enzymes for the Homeland Defense: Optimizing Phosphotriesterase for the Hydrolysis of Organophosphate Nerve Agents.Biochemistry
Fan F, Williams HJ, Boyer JG, Graham TL, Zhao H, Lehr R, Qi H, Schwartz B, Raushel FM & Meek TD (2012)On the catalytic mechanism of human ATP citrate lyase. Biochemistry 51: 5198-211
Bigley AN & Raushel FM (2012) Catalytic mechanisms for phosphotriesterases. Biochim Biophys Acta
Hobbs ME, Malashkevich V, Williams HJ, Xu C, Sauder JM, Burley SK, Almo SC & Raushel FM (2012)Structure and Catalytic Mechanism of LigI: Insight into the Amidohydrolase Enzymes of cog3618 and Lignin Degradation. Biochemistry
Xiang DF, Kolb P, Fedorov AA, Xu C, Fedorov EV, Narindoshivili T, Williams HJ, Shoichet BK, Almo SC & Raushel FM (2012) Structure-based function discovery of an enzyme for the hydrolysis of phosphorylated sugar lactones. Biochemistry 51: 1762-73
2011
Kamat SS, Williams HJ & Raushel FM (2011) Intermediates in the transformation of phosphonates to phosphate by bacteria. Nature 480: 570-3
Gerlt JA, Allen KN, Almo SC, Armstrong RN, Babbitt PC, Cronan JE, Dunaway-Mariano D, Imker HJ, Jacobson MP, Minor W, Poulter CD, Raushel FM, Sali A, Shoichet BK & Sweedler JV (2011) The Enzyme Function Initiative. Biochemistry 50: 9950-62
Kamat SS, Holmes-Hampton GP, Bagaria A, Kumaran D, Tichy SE, Gheyi T, Zheng X, Bain K, Groshong C, Emtage S, Sauder JM, Burley SK, Swaminathan S, Lindahl PA & Raushel FM (2011) The catalase activity of diiron adenine deaminase. Protein Sci 20: 2080-94
Goble AM, Fan H, Sali A & Raushel FM (2011) Discovery of a cytokinin deaminase. ACS Chem Biol 6: 1036-40
Goble AM, Zhang Z, Sauder JM, Burley SK, Swaminathan S & Raushel FM (2011) Pa0148 from Pseudomonas aeruginosa catalyzes the deamination of adenine. Biochemistry 50: 6589-97
Hitchcock DS, Fedorov AA, Fedorov EV, Dangott LJ, Almo SC & Raushel FM (2011) Rescue of the orphan enzyme isoguanine deaminase. Biochemistry 50: 5555-7
Hall RS, Fedorov AA, Xu C, Fedorov EV, Almo SC & Raushel FM (2011) Three-dimensional structure and catalytic mechanism of Cytosine deaminase. Biochemistry 50: 5077-85
Kamat SS, Fan H, Sauder JM, Burley SK, Shoichet BK, Sali A & Raushel FM (2011) Enzymatic deamination of the epigenetic base N-6-methyladenine. J Am Chem Soc 133: 2080-3
Raushel FM (2011) Chemical biology: Catalytic detoxification. Nature 469: 310-1
Kamat SS, Bagaria A, Kumaran D, Holmes-Hampton GP, Fan H, Sali A, Sauder
JM, Burley SK, Lindahl PA, Swaminathan
S & Raushel FM (2011) Catalytic
mechanism and three-dimensional structure of adenine deaminase. Biochemistry
50: 1917-27
2010
M.E. Meyer, J.A. Gutierrez,
F.M. Raushel, N.G. Richards, “A
conserved glutamate controls the commitment to acyl-adenylate
formation in asparagine synthetase” Biochemistry 49,
9391-9401 (2010).
P.C. Tsai, A. Bigley, Y. Li, E.
Ghanem, C.L. Cadieux, S.A. Kasten, T.E. Reeves, D.M. Cerasoli, F.M.
Raushel, “Stereoselective
hydrolysis of organophosphate nerve agents by the bacterial
phosphotriesterase” Biochemistry 49,
7978-7987 (2010).
P.C. Tsai, Y. Fan, J. Kim, L. Yang, S.C.
Almo, Y.Q. Gao, F.M. Raushel, “Structural determinants for the
stereoselective hydrolysis of chiral substrates by phosphotriesterase”
Biochemistry 49, 7988-7997 (2010).
D.F. Xiang, Y. Patskovsky, C. Xu, A.A.
Fedorov, E.V. Fedorov, A.A. Sisco, S.K. Burley, S.C. Almo, F.M.
Raushel, “Functional identification and structure determination of two
novel prolidases from cog1228 in the amidohydrolase superfamily” Biochemistry
49, 6791-6803 (2010).
R.S. Hall, R. Agarwal, D. Hitchcock, J.M.
Sauder, S.K. Burley, S. Swaminathan, F.M. Raushel, “Discovery and
structure determination of the orphan enzyme isoxanthopterin
deaminase” Biochemistry 49,
4374-4382 (2010).
L. Lund, Y. Fan, Q. Shao, Y.Q. Gao, F.M.
Raushel, “Carbamate transport in carbamoyl phosphate synthetase: a
theoretical and experimental investigation” J Am Chem Soc
132, 3870-3878 (2010).
R.S. Hall, A.A. Fedorov, R. Marti-Arbona,
E.V. Fedorov, P.Kolb, J.M. Sauder, S.K. Burley, B.K. Shoichet, S.C.
Almo, F.M. Raushel, “The hunt for 8-oxoguanine deaminase” J
Am Chem Soc 132, 1762-1763 (2010).
J.A. Cummings, T.T. Nguyen, A.A. Fedorov, P.
Kolb, C. Xu, E.V. Fedorov, B.K. Shoichet, D.P Barondeau, S.C. Almo,
F.M. Raushel, “Structure, mechanism, and substrate profile for
Sco3058: the closest bacterial homologue to human renal dipeptidase” Biochemistry 49, 611-622 (2010).
2009
D.F. Xiang, P. Kolb, A.A.
Fedorov, M.M. Meier, L.V. Fedorov, T.T. Nguyen, R. Sterner, S.C. Almo,
B.K. Shoichet, F.M. Raushel, “Functional annotation and
three-dimensional structure of Dr0930 from Deinococcus
radiodurans, a close relative of phosphotriesterase in the
amidohydrolase superfamily” Biochemistry 10,
2237-2247 (2009).
U. Pieper, R. Chiang, J.J.
Seffernick, S.D. Brown, M.E. Glasner, L. Kelly, N. Eswar, J.M. Sauder,
J.B. Bonanno, S. Swaminathan, S.K. Burley, X. Zheng, M.R. Chance, S.C.
Almo, J.A. Gerlt, F.M. Raushel, M.P. Jacobson, P.C. Babbitt, A. Sali,
“Target selection and annotation for the structural genomics of the
amidohydrolase and enolase superfamilies” J Struct Funct
Genomics 10, 107-125
(2009).
D.F. Xiang, Y. Patskovsky, C.
Xu, A.J. Meyer, J.M. Sauder, S.K. Burley, S.C. Almo, F.M. Raushel,
“Functional identification of incorrectly annotated prolidases from
the amidohydrolase superfamily of enzymes” Biochemistry 48, 3730-3742 (2009).
D.F. Xiang, C. Xu, D. Kumaran,
A.C. Brown, J.M. Sauder, S.K. Burley, S. Swaminathan, F.M. Raushel,
“Functional annotation of two new carboxypeptidases from the
amidohydrolase superfamily of enzymes” Biochemistry 48,
4567-4576 (2009).
J.A. Cummings, A.A. Fedorov, C.
Xu, S. Brown, E. Fedorov, P.C. Babbitt, S.C. Almo, F.M. Raushel,
“Annotating enzymes of uncertain function: the deacylation of D-amino
acids by members of the amidohydrolase superfamily” Biochemistry
48, 6469-6481 (2009).
Y. Fan, L. Lund, Q. Shao, Y. Q.
Gao, F.M. Raushel, “A combined theoretical and experimental study of
the ammonia tunnel in carbamoyl phosphate synthetase” JACS
131, 10211-10219 (2009).
T.T. Nguyen, A.A. Fedorov, L.
Williams, E.V. Fedorov, Y. Li, C. Xu, S.C. Almo, F.M. Raushel, “The
mechanism of the reaction catalyzed by uronate isomerase illustrates
how an isomerase may have evolved from a hydrolase within the
amidohydrolase superfamily” Biochemistry 48,
8879-8890 (2009).
2008
J. Kim, P.C. Tsai, S.L. Chen,
F. Himo, S.C. Almo, F.M. Raushel, “Structure of diethyl phosphate
bound to the binuclear metal center of phosphotriesterase” Biochemistry
47, 9497-9504 (2008).
T.
T. Nguyen, S. Brown, A. A. Fedorov, E. V. Fedorov, P. C. Babbitt, S.
C. Almo, and F. M. Raushel, "At the Periphery of the Amidohydrolase
Superfamily: Bh0493 from Bacillus
halodurans Catalyzes the
Isomerization of D-Galacturonate to D-Tagaturonate"
Biochemistry, 47, 1194-1206 (2008).
Y.
Fan, L. Yang, L. Lund, F. M. Raushel, and Y. Q. Gao, "The Mechanism for the Transport of Ammonia
within Carbamoyl Phosphate Synthetase Determined by Molecular
Dynamics Simulations" Biochemistry 47,
2935-2944 (2008).
L.
Williams, F. Fan, J. S. Blanchard, and F. M. Raushel, "Positional Isotope Exchange Analysis of the
Mycobacterium smegmatis Cysteine Ligase (MshC)"
Biochemistry 47, 4843-4850 (2008).
R.
Sterner, R. Merkl, F. M. Raushel, "Computational Design of Enzymes"
Chemistry and Biology 15, 421-423 (2008).
R.
Tyagi, S. Eswaramoorthy, S. K. Burley, F. M. Raushel, S. Swaminathan,
"A Common Catalytic Mechanism for Proteins of
HutI Family" Biochemistry 47, 5608-5615
(2008).
R. Z. Liao, J. G. Yu, F. M. Raushel, and F. Himo, "Theoretical Investigation of the Reaction Mechanism of the Dinuclear Zinc Enzyme Dihydroorotase" Chemistry: A European Journal 14, 4287-4292 (2008).
2007
Y.
Li, F.M. Raushel, “Differentiation of chiral phosphorus enantiomers by
P and H NMR spectroscopy using amino acid derivatives as chemical
solvating agents” Tetrahedron Asymmetry 18,
1391-1397 (2007).
V.
Fresquet, L. Williams, F.M. Raushel, “Partial randomization of the
four sequential amidation reactions catalyzed by cobyric acid
synthetase with a single point mutation” Biochemistry 46, 13983-13993 (2007).
E.
Ghanem, Y. Li, C. Xu, F.M. Raushel, “Characterization of a
phosphodiesterase capable of hydrolyzing EA 2192, the most toxic
degradation product of the nerve agent VX” Biochemistry 46, 9032-40 (2007).
J.C.
Hermann,
R. Marti-Arbona, A.A. Fedorov, E. Fedorov, S.C. Almo, B.K. Shoichet,
F.M. Raushel, “Structure-based activity prediction for an enzyme of
unknown function” Nature 448, 775-778
(2007).
R.S.
Hall,
S. Brown, A.A. Fedorov, E. Fedorov, C. Xu, P.C. Babbitt, S.C. Almo,
F.M. Raushel, “Structural diversity within the mononuclear and
binuclear active sites of N-acetyl-D-glucosamine-6-phosphate
deacetylase” Biochemistry 46,
7953-7962 (2007).
R.S.
Hall,
D.F. Xiang, C. Xu, F.M. Raushel, “N-Acetyl-D-glucosamine-6-phosphate
deacetylase: substrate activation via a single divalent metal ion” Biochemistry 46, 7942-7952 (2007).
C.R.
Samples,
F.M. Raushel, V.J. DeRose, “Activation of
the binuclear metal center through formation of
phosphotriesterase-inhibitor complexes” Biochemistry 46, 3435-3442 (2007).
L.
Williams, V. Fresquet, P.J. Santander, F.M. Raushel, “The multiple
amidation reactions catalyzed by Cobyric acid synthetase from Salmonella
typhimurium are sequential and dissociative” JACS 129, 294-295 (2007).
G.
Proteasa, Y.R. Tahboub, S. Galijasevic, F.M. Raushel, H.M. Abu-Soud,
“Kinetic evidence supports the existence of two halide binding sites
that have a distinct impact on the heme iron microenvironment in
myeloperoxidase” Biochemistry 46,
398-405 (2007).
2006
R.
Marti-Arbona, C. Xu, S. Steele, A. Weeks, G. F. Kuty, C. M. Seibert,
and F. M. Raushel, “Annotating Enzymes of Unknown Function: N-Formimino-L-Glutamate Deiminase is a Member of the
Amidohydrolase Superfamily” Biochemistry 45,
1997-2005 (2006).
Y.
I. Korpan, F. M. Raushel, A. A. Nazarenko, A. P. Soldatkin, N.
Jaffrezic-Renault, C. Martelet, “Sensitivity and Specificity Improvement of
ISFET-based Biosensor for Potato Glycolalkaloids Detection”
Journal of Agricultural and Food Chemistry 54, 707-712
(2006).
C.
Xu, R. Hall, J. Cummings, and F. M. Raushel, “Tight Binding Inhibitors of N-Acyl Amino
Sugar and Amino Acid Deacetylases” Journal of the
American Chemical Society 128, 4244-4255 (2006).
Sakai,
W.
S. Yew, L. Song, D. F. Xiang, C. Xu, F. M. Raushel and J. A. Gerlt, “Evolution of Enzymatic Activities in the
Enolase Superfamily: N-Succinylamino Acid Racemase and a New
Pathway for the Irreversible Conversion of D- to L-Amino
Acids” Biochemistry 45, 4455-4462
(2006).
L.
Williams, T. Nguyen, Y. Li, T. N. Porter, and F. M. Raushel, “Uronate Isomerase: A Nonhydrolytic Member of
the Amidohydrolase Superfamily with an Ambivalent Requirement for
a Divalent Metal Ion” Biochemistry 45,
7453-7462 (2006).
A.
Weeks, L. Lund, and F. M. Raushel, “Tunneling of Intermediates in
Enzyme-Catalyzed Reactions” Current Opinion in
Chemical Biology 10, 465-472 (2006).
R.
Marti-Arbona and F. M. Raushel, “Mechanistic Characterization of N-Formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa” Biochemistry
45, 14256-14262 (2006).
J.
C. Hermann, E. Ghanem, Y. Li, F. M. Raushel, J. J. Irwin, and B. K.
Shoichet, “Predicting Substrates by Docking High-Energy
Intermediates to Enzyme Structures” Journal of the
American Chemical Society 128, 15882-15891
(2006).
A.
Nowlan, Y. Li, T. Evans, J. Carpenter, E. Ghanem, J. C. Hermann, B. K.
Shoichet, and F. M. Raushel, “Resolution of Chiral Phosphate, Phosphonate,
and Phosphinate Esters by an Enantioselective Enzyme Library”
Journal of the American Chemical Society 128,
15892-15902, (2006).
R. Marti-Arbona, J.B. Thoden,
H.M. Holden, F.M. Raushel, “Functional
significance of Glu-77 and Tyr-137 within the active site of
isoaspartyl dipeptidase” Bioorg Chem 33,
448-458 (2005).
2005
Y. Li, F.M. Raushel,
“Inhibitors designed for the active site of dihydroorotase” Bioorg
Chem 33, 470-483 (2005).
J.J.
Irwin,
F.M. Raushel, B.K. Shoichet, “Virtual screening against metalloenzymes
for inhibitors and substrates” Biochemistry 44,
12316-12328 (2005).
C.R.
Samples,
T. Howard, F.M Raushel, V.J. DeRose, “Protonation of the binuclear
metal center within the active site of phosphotriesterase” Biochemistry
44, 11005-11013 (2005).
E.
Ghanem, F.M. Raushel, “Detoxification of organophosphate nerve agents
by bacterial phosphotriesterase” Toxicol Appl Pharmacol 207, 459-470 (2005).
R.
Marti-Arbona, V. Fresquet, J.B. Thoden, M.L.Davis, H.M. Holden, F.M.
Raushel, “Mechanism of the reaction catalyzed by isoaspartyl
dipeptidase from Escherichia coli” Biochemistry
44, 7115-7124 (2005).
C.M. Seibert, F.M. Raushel, “Structural and catalytic diversity within the amidohydrolase superfamily” Biochemistry 44, 6393-6391 (2005).
2004
V.
Fresquet, J. B. Thoden, H. M. Holden, and F. M. Raushel, "Kinetic Mechanism of Asparagine Synthetase
from Vibro Cholerae"
Bioorganic Chemistry 32, 63-75 (2004).
J.
Kim, and F. M. Raushel, "Access to the carbamate tunnel of carbamoyl
phosphate synthetase" Archives of Biochemistry and
Biophysics 425, 33-41 (2004).
J.
Kim, and F. M. Raushel, "Perforation of the Tunnel Wall in Carbamoyl
Phosphate Synthetase Derails the Passage of Ammonia between
Sequential Active Sites" Biochemistry 43,
5334-5340 (2004).
S.
D. Aubert, Y. Li, and F. M. Raushel, "Mechanism for the Hydrolysis of
Organophosphates by the Bacterial Phosphotriesterase" Biochemistry
43, 5707-5715 (2004).
S.
H. Hong and F. M. Raushel, "Control of Stereoselectivity in
Phosphotriesterase" Methods in Enzymology 388, 256-266
(2004).
Y.
Li, S. D. Aubert, E. G. Maes, and F. M. Raushel, "Enzymatic Resolution of Chiral Phosphinate
Esters" Journal of the American Chemical Society 126,
29, 8888-8889 (2004).
M.-S.
Park,
C. M. Hill, Y. Li, R. K. Hardy, H. Khanna, Y. H. Khang, F. M. Raushel,
"Catalytic properties of the PepQ prolidase from Escherichia coli"
Archives of Biochemistry and Biophysics 429, 224-230
(2004).
V.
Fresquet, L. Williams, and F. M. Raushel, "Mechanism of Cobyrinic Acid a,c-Diamide
Synthetase from Salmonella
typhimurium LT2"
Biochemistry, 43, 33, 10619-10627 (2004).
T.
N. Porter, Y. Li, and F. M. Raushel, "Mechanism of Dihydroorotase Reaction"
Biochemistry 43, 51, 16285-16292 (2004).
J.
B. Thoden, Z. Huang, J. Kim, F. M. Raushel, and H. M. Holden, "Long Range Allosteric Transitions in
Carbamoyl Phosphate Synthetase" Protein Science
13, 2398-2405 (2004).
H.
M. Abu-Soud, F. M. Raushel, and S., "A Novel Multistep Mechanism for Oxygen
Binding to Ferrous Hemoproteins: Rapid Kinetic Analysis of
Ferrous-Dioxy Myeloperoxidase (Compound III) Formation"
Biochemistry 43, 11589-11595 (2004).
2003
C.M.
Hill,
W.S. Li, J.B. Thoden, H.M. Holden, F.M. Raushel, “Enhanced degradation
of chemical warfare agents through molecular engineering of the
phosphotriesterase active site” JACS 125,
8990-8991 (2003).
J.B.
Garrett,
L.S. Mullins, F.M. Raushel, “Effect of linker sequence on the
stability of circularly permuted variants of ribonuclease T1” Bioorg
Chem 31, 412-424 (2003).
K.T.
Lum,
H.J. Huebner, Y. Li, T.D. Phillips, F.M. Raushel, “Organophosphate
nerve agent toxicity in Hydra attenuate” Chem Res Toxicol
16, 953-957, (2003).
F.M.
Raushel,
J.B. Thoden, H.M. Holden, “Enzymes with molecular tunnels” Acc
Chem Res 36, 539-548 (2003).
Y.
Li, S.D. Aubert, F.M. Raushel, “Operational control of
stereoselectivity during the enzymatic hydrolysis of racemic
organophosphorus compounds” JACS 125,
7526-7527 (2003).
L.
Williams, R. Zheng, J.S. Blanchard, F.M. Raushel, “Positional isotope
exchange analysis of the pantothenate synthetase reaction” Biochemistry
42, 5108-5113 (2003).
J.B.
Thoden,
R. Marti-Arbona, F.M. Raushel, H.M. Holden, “High-resolution X-ray
structure of isoaspartyl dipeptidase from Escherichia
coli” Biochemistry 42,
4874-4882 (2003).
J.B.
Thoden,
J. Kim, F.M. Raushel, H.M. Holden, “The
catalytic mechanism of galactose mutarotase” Protein
Science 12, 1051-1059 (2003).
J.A.
Gerlt,
F.M. Raushel, “Evolution of function in (beta/alpha)8-barrel enzymes”
Current Opinions in Chemical Biology 7,
252-264 (2003).
B.
W. Miles, J. B. Thoden, H. M. Holden, and F. M. Raushel, "Inactivation of the
Amidotransferase Activity of Carbamoyl Phosphate Synthetase by the
Antibiotic Acivicin" Journal of Biological
Chemistry 277, 4368-4373 (2002).
O.
Pierrat and F. M. Raushel, "A Functional Analysis of the Allosteric
Nucleotide Monophosphate binding Site of Carbamoyl Phosphate
Synthetase" Archives of Biochemistry & Biophysics 400,
34-42 (2002).
O.
Pierrat, F. Javid-Majd and F. M. Raushel, "Dissection of the Conduit
for Allosteric Control of Carbamoyl Phosphate Synthetase by Ornithine"
Archives of Biochemistry & Biophysics 400,
26-33 (2002).
W.
Li, Y. Li, C. M. Hill, K. T. Lum, and F. M. Raushel, "Enzymatic Synthesis of
Chiral Organophosphothioates from Prochiral Precursors"
Journal of American Chemical Society 124,
3498-3499 (2002).
F.
M. Raushel, "Bacterial Detoxification
of Organophosphate Nerve Agents" Current Opinion in
Microbiology 5, 288-295 (2002).
J.
Kim, S. Howell, X. Huang, and F. M. Raushel, "Structural Defects
within the Carbamate Tunnel of Carbamoyl Phosphate Synthetase"
Biochemistry 41, 12575-12581 (2002).
J.
Thoden, X. Huang, F. M. Raushel, and H. M. Holden, "Carbamoyl Phosphate
Synthetase: Creation of an Escape Route for Ammonia" Journal
of Biological Chemistry 277, 39722-39727 (2002).
J. B. Thoden, J. Kim, F. M. Raushel, and H. M.
Holden, "Structural and Kinetic
Studies of Sugar Binding to Galactose Mutarotase from Lactococcus lactis" Journal
of Biological Chemistry 277, 45458-45465 (2002).
2001
M.A.
Anderson,
H. Shim, F.M. Raushel, W.W.Cleland, “Hydrolysis of phosphotriesters:
determination of transition states in parallel reactions by heavy-atom
isotope effects” JACS 123, 9246-9253
(2001).
M.M. Benning, H. Shim,
F.M.Raushel, H.M Holden, “High resolution
X-ray structures of different metal-substituted forms of
phosphotriesterase from Pseudomonas diminuta” Biochemistry
40, 2712-2722 (2001).
M. Chen-Goodspeed, M.A. Sogorb,
F. Wu, S.B. Hong, F.M. Raushel, “Structural determinants of the
substrate and stereochemical specificity of phosphotriesterase” Biochemistry
40, 1325-1331 (2001).
M. Chen-Goodspeed, M.A. Sogorb,
F. Wu, F.M. Raushel, “Enhancement, relaxation, and reversal of the
stereoselectivity for phosphotriesterase by rational evolution of
active site residues” Biochemistry 40,
1332-1339 (2001).
C.M. Hill, W.S. Li, T.C. Cheng,
J.J. DeFrank, F.M. Raushel, “Stereochemical
specificity of organophosphorus acid anhydrolase toward p-nitrophenyl
analogs of soman and sarin” Bioorg Chem 29,
27-35 (2001).
X. Huang, H.M Holden, F.M.
Raushel, “Channeling of substrates and intermediates in
enzyme-catalyzed reactions” Annu Rev Biochem 70,
149-180 (2001).
J. Kim, F.M. Raushel,
“Allosteric control of the oligomerization of carbamoyl phosphate
synthetase from Escherichia coli” Biochemistry
40, 11030-11036 (2001).
W.S. Li, K.T. Lum, M.
Chen-Goodspeed, M.A. Sogorb, F.M. Raushel, “Stereoselective
detoxification
of chiral sarin and soman analogues by phosphotriesterase” Bioorg
Med Chem 9, 2083-2091 (2001).
J.B. Thoden, G.N. Phillips Jr.,
T.M. Neal, F.M Raushel, H.M. Holden, “Molecular structure of
dihydroorotase: a paradigm for catalysis through the use of a
binuclear metal center” Biochemistry 40,
6989-6997 (2001).
F. Javid-Majd, L. S. Mullins,
F. M. Raushel, and M. A. Stapleton, “The Differentially Conserved Residues of
Carbamoyl Phosphate Synthetase” Journal of
Biological Chemistry 275, 5073-5080 (2000).
2000 and Before
X. Huang and F. M. Raushel, “An Engineered Blockage within the Ammonia
Tunnel in Carbamoyl Phosphate Synthetase Prevents of the Use of
Glutamine as a Substrate but not Ammonia” Biochemistry
39, 3240-3247 (2000).
M. Raushel and H. M. Holden,
“Phosphotriesterase: An Enzyme in Search of Its Natural Substrate” Advances
in Enzymology 74, 51-93 (2000).
B. W. Miles and F. M. Raushel,
“Synchronization of the Three Reaction
Centers within Carbamoyl Phosphate Synthetase” Biochemistry
39, 5051-5056 (2000).
H. Shim and F. M. Raushel, “Self-Assembly of the Binuclear Metal Center
of Phosphotriesterase” Biochemistry 39,
7357-7364 (2000).
C. Hill, F. Wu, T. Chen., J.
DeFrank, and F. M. Raushel, “Substrate and Stereochemical Specificity of
the Organophosphorus Acid Anhydrolase from Alteromonas sp. JD6.5
Toward p-Nitrophenyl
Phosphotriesters" Bioorganic and Medicinal
Chemistry Letters 10, 1285-1288. (2000).
V.L. Healy, L.S. Mullins, X.
Li, S.E. Hall, F.M. Raushel, and C.T. Walsh, “D-Ala-D-X Ligases: Evaluation of D-Alanyl
Phosphate Intermediate by MIX, PIX and Rapid Quench Studies”
Chemistry and Biology 7, 505-514 (2000).
X. Huang and F. M. Raushel, “Role of the Hinge Loop Linking the N- and
C-Terminal Domains of the Amidotransferase Subunit of Carbamoyl
Phosphate Synthetase” Archives of Biochemistry and
Biophysics 380, 174-180 (2000).
X. Huang and F. M. Raushel, “Restricted
Passage of Reaction Intermediates Through the Ammonia Tunnel of
Carbamoyl Phosphate Synthetase” Journal of
Biological Chemistry 275, 26233-26240 (2000).
W. Zhu, F. Wu, F. M. Raushel,
and G. Vigh, “Capillary Electrophoretic Separation of the
Enantiomers of Organophosphates with a Phosphorus Stereogenic
Center Using Octakis(2,3-diacetyl-6-sulfato)-g-cyclodextrin as Resolving Agent”
Journal of Chromatography 895, 247-254 (2000).
W. Zhu, W.-S. Li, F. M.
Raushel, and G. Vigh, “Experimental Verification of a Predicted,
Hitherto Unseen Separation Selectivity Pattern in the Capillary
Electrophoretic Separation of Nonchared Enantiomers by
Octakis(2,3-Diacetyl-6-Sulfato)--Cylclodextrin” Electrophoresis
21, 3249-3256 (2000).
M. M. Benning, S. B. Hong, F.
M. Raushel and H. M. Holden, “The Binding of Substrate Analogs to
Phosphotriesterase” Journal of Biological Chemistry
275, 30546-30550 (2000).
F. Wu, W.-S. Li, M.
Chen-Goodspeed, M. Sogorb, and F. M. Raushel, “Rationally Engineered Mutants of
Phosphotriesterase for Preparative Scale Isolation of Chiral
Organophosphates” Journal of American Chemical
Society 122, 10206-10207 (2000).
J.
B. Thoden, F. M. Raushel, M. M. Benning, I. Rayment, and H. M. Holden,
"The Structure of Carbamoyl Phosphate Synthetase Determined to 2.1 Å
Resolution" Acta Crystallographic Part D 55, 8-24
(1999).
S.-B
Hong
and F. M. Raushel, "Stereochemical
Constraints on the Substrate Specificity of Phosphotriesterase"
Biochemistry 38, 1159-1165 (1999).
B. L. Braxton, L. S. Mullins, F. M. Raushel
and G. D. Reinhart, "Allosteric Dominance in
Carbamoyl Phosphate Synthetase" Biochemistry 38,
1394-1401 (1999).
M.
Raushel, L. S. Mullins and G. Gibson, "Detection of Intermediates in
the Carbamoyl Phosphate Synthetase Reaction" in Enzyme Mechanisms
(P. A. Frey and D. B. Northrop, eds.) IOS Press, Amsterdam,
Netherlands pp. 61-71 (1999).
J.
B. Thoden, G. Wesenberg, F. M. Raushel and H. M. Holden, "Carbamoyl Phosphate
Synthetase: Closure of the B-domain as a Result of Nucleotide
Binding" Biochemistry 38, 2347-2357
(1999).
L.
M. Mullins and F. M. Raushel, "Channeling of Ammonia
Through the Intermolecular Tunnel Contained within Carbamoyl
Phosphate Synthetase" Journal of American Chemical
Society 121, 3803-3804 (1999).
F.M.
Raushel,
J. B. Thoden and H. M. Holden, "The Amidotransferase
Family of Enzymes: Molecular Machines for the Production and
Delivery of Ammonia" Biochemistry 38,
7891-7899 (1999).
S.
B. Hong and F. M. Raushel, "Stereochemical
Preferences for Chiral Substrates by the Bacterial
Phosphotriesterase" Chemico-Biological Interactions
119, 225-234 (1999).
T.
O. Baldwin, M. M. Zeigler and F. M. Raushel, "Structure, Folding, and
Mechanism of Bacterial Luciferase, in Bioluminescence and
Chemiluminescence: Perspective for the 21st Centrury.
(Roda, K. A., Pazzagli, M., Kricka, L. J. and Stanley, P. E., eds.)
John Wiley & Sons, England pp. 376-379 (1999).
K.
Tuovinen, E. Kaliste-Korhonen, F. M. Raushel and O. Hanninen, "Success
of Pyridostigmine, Physostigmine, Eptastigmine and Phosphotriesterase
Treatments in Acute Sarin Intoxication" Toxicology 134,
169-178 (1999).
J.
B Thoden, F. M. Raushel, G. Wesenburg and H. M. Holden, "The Binding of Inosine
Monophosphate to E. coli Carbamoyl Phosphate Synthetase"
Journal of Biological Chemistry 274, 22502-22507
(1999).
S.B.
Hong
and F. M. Raushel, "Stereochemical Constraints on the Catalytic
Hydrolysis of Organosphosphate Nerve Agents by Phosphotriesterase" Phosphorus,
Sulfur, and Silicon and the Related Elements 144,
521-524 (1999).
M.
Chen-Goodspeed, J. Vanhooke, H. M. Holden and F. M. Raushel, "Kinetic
Mechanism of Kanamycin Nucleotidyltransferase from Stapylococcus
Aureus" Bioorganic Chemistry 27, 395-408 (1999).
X. Huang and F. M. Raushel, "Deconstruction of the
Catalytic Array within the Amidotransferase Subunit of Carbamoyl
Phosphate Synthetase" Biochemistry 38,
15909-15914 (1999).
J. B. Thoden, X. Huang, F. M. Raushel, and H.
M. Holden, "Carbamoyl Phosphate
Synthetase: Snapshots Along the Reaction Pathway" Biochemistry
38, 16158-16166 (1999).
H. M. Holden, F. M. Raushel, and J. B. Thoden,
"Carbamoyl Phosphate
Synthetase: An Amazing Biochemical Odyssey from Substrate to
Product" Cellular and Molecular Life Sciences 56,
507-522 (1999).
W.
A. Francisco, H. M. Abu-Soud, A. DeMonte, D. Singleton, T. O. Baldwin
and F. M. Raushel, "Deuterium Kinetic
Isotope Effect and the Mechanism of the Bacterial Luciferase
Reaction" Biochemistry 37, 2596-2606
(1998).
J.
B. Thoden, S. G. Miran, J. C. Phillips, A J. Howard, F. M. Raushel and
H. M. Holden, "Carbamoyl Phosphate
Synthetase: Caught in the Act of Glutamine Hydrolysis"
Biochemistry 37, 8825-8831 (1998).
H. Shim, S. B. Hong and F. M. Raushel, "Hydrolysis of
Phosphodiesters through Transformation of the Bacterial
Phosphotriesterase" Journal of Biological Chemistry
273, 17445-17450 (1998).
F.M.
Raushel,
L. S. Mullins and G. E. Gibson, "A Stringent Test for the
Nucleotide Switch Mechanism of Carbamoyl Phosphate Synthetase"
Biochemistry 37, 10272-10278 (1998).
F.
M. Raushel, J. B. Thoden, G. D. Reinhart and H. M. Holden, "Carbamoyl Phosphate
Synthetase: A Crooked Path from Substrates to Products"
Current Opinion in Chemical Biology 2, 624-632 (1998).
B. W. Miles, J. A. Banzon, and F. M. Raushel,
"Regulatory Control of
the Amidotransferase Domain of Carbamoyl Phosphate Synthetase"
Biochemistry 37, 16773-16779 (1998).
H. M. Holden, J. B. Thoden, and F. M. Raushel,
"Carbamoyl Phosphate
Synthetase: A Tunnel Runs Through It" Current
Opinion in Structural Biology 8, 679-685 (1998).
G.
W. Gibson, L. S. Mullins, and F. M. Raushel, "Carbamoyl Phosphate
Synthetase from Escherichia coli does not Catalyze the
Dehydration of Bicarbonate to Carbon Dioxide" Bioorganic Chemistry
26, 255-268 (1998).
J.
M. Kuo, M. Y. Chae and F. M. Raushel, "Perturbations to the
Active Site of Phosphotriesterase" Biochemistry 36,
1982-1988 (1997).
J. B. Thoden, H. M. Holden, G. Wesenberg, F.
M. Raushel and I. Rayment, "The Structure of
Carbamoyl Phosphate Synthetase: A Journey of 96: from Substrate to
Product" Biochemistry 36, 6305-6316
(1997).
S.
B. Hong, L. S. Mullins, H. Shim, and F. M. Raushel, "Mechanism-Based
Inhibitors for the Inactivation of the Bacterial
Phosphotriesterase" Biochemistry 36,
9022-9028 (1997).
L.
S. Mullins, S. B. Hong, G. E. Gibson, H. Walker, T. C. Stadtman and F.
M. Raushel, "Identification of a
Phosphorylated Enzyme Intermediate in the Catalytic Mechanism for
Selenophosphate Synthetase" Journal of American
Chemical Society 119, 6684-6685 (1997).
H. M. Abu-Soud, R. Batchui, F. M. Raushel and
D. J. Stuehr, "The Ferrous-dioxy
Complex of Neuronal Nitric-oxide Synthase" Journal
of Biological Chemistry 272, 17349-17353 (1997).
L. S. Mullins, C. N. Pace and F. M. Raushel, "Conformational Stability of Ribonuclease T1
Determined by Hydrogen-Deuterium Exchange" Protein
Science 6, 1387-1395 (1997).
S.
B. Hong and F. M. Raushel, "Inhibitors Directed Towards the Binuclear
Metal Center of Phosphotriesterase" Journal of Enzyme Inhibition 12,
191-203 (1997).
L.
M. Watkins, H. J. Mahoney, J. K. McCulloch and F. M. Raushel, "Augmented Hydrolysis of
DFP in Engineered Mutants of Phosphotriesterase" Journal
of Biological Chemistry 272, 25596-25627 (1997).
T.
O. Baldwin, M. M. Ziegler, A. C. Clark, J. F. Sinclair, F. M. Raushel,
I. Rayment, H. M. Holden, A. J. Fisher, T. B. Thompson, and J. B.
Thoden, "Structure and Folding of Bacterial Luciferase" in Flavins
and Flavoproteins (1996).
K.
J. Stevenson, V. Massey and C. H. Williams, eds) University of Calgary
Press, pp. 347-356 (1997).
L. M. Watkins, J. M. Kuo, M. Chen-Goodspeed
and F. M. Raushel, "A Combinatorial Library
for the Binuclear Metal Center of Bacterial Phosphotriesterase"
PROTEINS: Structure, Function and Genetics 29, 553-561
(1997).
W.
A. Francisco, H. M. Abu-Soud, R. Topgi, T. O. Baldwin and F. M.
Raushel, "Interaction of Bacterial Luciferase with
8-Substituted Flavin Mononucleotide Derivatives" Journal
of Biological Chemistry 271, 104-110 (1996).
J. B. Garrett, L. S. Mullins and F. M.
Raushel, "Are Turns Required for
the Folding of Ribonuclease T1" Protein Science 5,
204-211 (1996).
J. L. Vanhooke, M. M. Benning, F. M. Raushel,
and H. M. Holden, "Three-Dimensional
Structure of the Zinc-Containing Phosphotriesterase with the Bound
Substrate Analog Diethyl 4-Methylbenzylphosphonate" Biochemistry
35, 6020-6025 (1996).
K.
Tuovinen, E. Kaliste-Korhonen, F. Raushel, and O. Hanninen,
"Protection of Organophosphate-Inactivated Esterases with
Phosphotriesterase" Fundamental & Applied Pharmacology 31,
210-217 (1996).
J. L. Johnson and F. M. Raushel, "The Influence of
Primary Sequence Transpositions on the Folding Pathways of
Ribonuclease T1" Biochemistry 35,
10223-10233 (1996).
S.-B.
Hong
and F. M. Raushel, "Metal-Substrate
Interactions Facilitate the Catalytic Activity of the Bacterial
Phosphotriesterase" Biochemistry 35,
10904-10912 (1996).
B.
L. Braxton, L. S. Mullins, F. M. Raushel and G. D. Reinhart, "The Allosteric Effects
of Carbamoyl Phosphate Synthetase from E.
coli are Entropy-Driven"
Biochemistry 35, 11918-11924 (1996).
L.
C. Lo, C. H. Lo, K. D. Janda, D. B. Kassel and F. M. Raushel, "A Versatile Mechanism
Based Reaction Probe for the Direct Selection of Biocatalysts"
Bioorganic & Medicinal Chemistry Letters 6,
2117-2120 (1996).
M. A. Stapleton, F. Javid-Majd, M. F. Harmon,
B. A. Hanks, J. L. Grahmann, L. S. Mullins and F. M. Raushel, "Role of Conserved
Residues Within the Carboxy Phosphate Domain of Carbamoyl
Phosphate Synthetase" Biochemistry 35,
14352-14361 (1996).
F. Javid-Majd, M. A. Stapleton, M. F. Harmon,
B. A. Hanks, L. S. Mullins and F. M. Raushel, "Comparison of the
Functional Differences for the Homologous Residues Within the
Carboxy Phosphate and Carbamate Domains of Carbamoyl Phosphate
Synthetase" Biochemistry 35,
14362-14369 (1996).
K.
Tuovinen, E. Kaliste-Korhonen, F. M. Raushel and O. Hanninen,
"Eptastigmine-Phosphotriesterase Combination in DFP Intoxication" Toxicology
and Applied Pharmacology 140, 364-369 (1996).
K.
Tuovinen, E. Kaliste-Korhonen, F. M. Raushel and O. Hanninen,
"Phosphotriesterase, Pralidoxime-2-Chloride (2-PAM) and Eptastigmine
Treatments and Their Combinations in DFP Intoxication" Toxicology
& Applied Pharmacology 141, 555-560 (1996).
L.
S. Mullins and F. M. Raushel, "Positional Isotope Exchange as a Probe
of Enzyme Action" in Enzyme Kinetics and Mechanism, Part D (Ed. D. L.
Purich) Academic Press 249, 398-425 Methods in
Enzymology (1995).
J.
Thoden, F. M. Raushel, S. Mareya, D. R. Tomchick and I. Rayment,
"Crystallization and Preliminary X-ray Crystallographic Analysis of
Carbamoyl Phosphate Synthetase from Escherichia coli" Acta
Crystallographica, Part D 51, 827-829. (1995).
M.
Y. Chae, G. A. Omburo, P. A. Lindahl, and F. M. Raushel, "Utilization
of Copper as a Paramagnetic Probe for the Binuclear Metal Center of
Phosphotriesterase" Archives of Biochemistry & Biophysics 316,
765-772 (1995).
J.
A. Banzon, J. M. Kuo, B. W. Miles, D. R. Fischer, P. J. Stang and F.
M. Raushel, "Mechanism-Based
Inactivation of Phosphotriesterase by Reaction of a Critical
Histidine with a Ketene Intermediate" Biochemistry
34, 743-749 (1995).
J. A. Banzon, J. M. Kuo, D. R. Fischer, P. J.
Stang and F. M. Raushel, "Histidine-254 of
Phosphotriesterase is Essential for Inactivation with the Alkynyl
Phosphate Esters and Diethyl Pyrocarbonate" Biochemistry
34, 750-754 (1995).
J.
M. Kuo, L. S. Mullins, J. B. Garrett and F. M. Raushel, "Circular
Permutation of RNase T1 Through PCR Based Site-Directed Mutagenesis" Techniques
in Protein Chemistry VI (J. W. Crabb, ed) Academic Press pp.
333-340 (1995).
S. M. Mareya and F. M. Raushel, "Mapping the Functional
Domains of E. coli Carbamoyl Phosphate Synthetase Using
Limited Proteolysis" Bioorganic & Medicinal
Chemistry 3, 525-532 (1995).
Frank
M.
Raushel, "Structural and Mechanistic Investigations of Carbamoyl
Phosphate Synthetase from E. coli" Pathways to
Pyrimidines: An International Newsletter 3, 1-8 (1995).
A.
J. Fisher, F. M. Raushel, T. O. Baldwin and I. Rayment, "The Three-dimensional
Structure of Bacterial Luciferase from Vibrio
harveyi at 2.4 A
Resolution" Biochemistry 34, 6581-6586
(1995).
M.
M. Benning, J. M. Kuo, F. M. Raushel and H. M. Holden, "Three-Dimensional
Structure of the Binuclear Metal Center of Phosphotriesterase"
Biochemistry 34, 7973-7978 (1995).
S.-B.
Hong,
J. M. Kuo, L. S. Mullins and F. M. Raushel, "CO2 is
Required for the Assembly of the Binuclear Metal Center of
Phosphotriesterase" Journal of American Chemical
Society 117, 7581-7582 (1995).
R.
M. Czerwinski, S. M. Mareya and F. M. Raushel, "Regulatory Changes in
the Control of Carbamoyl Phosphate Synthetase Induced by
Truncation and Mutagenesis of the Allosteric Binding Domain"
Biochemistry 34, 13920-13927 (1995).
L.
S. Mullins, M. Y. Chae and F. M. Raushel, "Phosphorus-31 NMR
Relaxation Studies of Diethyl p-Methoxyphenyl Phosphate Bound to
Phosphotriesterase" Bioorganic and Medicinal
Chemistry Letters 5, 3067-3072 (1995).
T. O. Baldwin, J. A. Christopher, F. M.
Raushel, J. F. Sinclair, M. M. Zeigler, A. J. Fisher and I. Rayment, "Structure of Bacterial
Luciferase" Current Opinion in Structural Biology 5,
798-809 (1995).
K.
Tuovinen, E. Kaliste-Korhonen, F. M. Raushel and O. Hanninen, "Phosphotriesterase - A
Promising Candidate for use in Detoxification of Organophosphates"
Fundamental &. Applied Toxicology 23, 578-584
(1994).
W.
A. Francisco, H. M. Abu-Soud, A. C. Clark, F. M. Raushel and T. O.
Baldwin, "Kinetic Mechanism of the Bacterial Luciferase Reaction" in Flavins
and Flavoproteins, 1993 (K. Yagi, ed) Walter Gruyter & Co.
Berlin (1994).
S. M. Mareya and F. M. Raushel, "A Molecular Wedge for
Triggering the Amidotransferase Activity of Carbamoyl Phosphate
Synthetase" Biochemistry 33, 2945-2950
(1994).
J. M. Kuo and F. M. Raushel, "Identification of the
Histidine Ligands to the Binuclear Metal Center of
Phosphotriesterase by Site-Directed Mutagenesis" Biochemistry
33, 4265-4272 (1994).
L.
S. Mullins, K. Wesseling, J. M. Kuo, J. B. Garrett, and F. M. Raushel,
"Transposition of
Protein Sequences: Circular Permutation of Ribonuclease T1"
Journal of American Chemical Society 116, 5529-5233
(1994).
L.
Pei, G. Omburo, W. D. McGuinn, I. Perikovics, K. Dave, F. M. Raushel,
J. R. Wild, J. R. DeLoach and J. L. Way, "Encapsulation of
Phosphotriesterase Within Murine Erythrocytes" Toxicology &
Applied Pharmacology 124, 296-301 (1994).
M.
Y. Chae, J. F. Postula, and F. M. Raushel, "Stereospecific
Enzymatic Hydrolysis of Phosphorus-Sulfur Bonds in Chiral
Organophosphate Triesters" Bioorganic &
Medicinal Chemistry Letters 4, 1473-1478 (1994).
W.
A. Francisco, H. M. Abu-Soud, F. M. Raushel and T. O. Baldwin,
"Studies on the Kinetic Mechanism of the Bacterial
Luciferase-Catalyzed Reaction" in Bioluminescence and
Chemiluminescence (A. A. Szalay, L. J. Kricka, and P. Stanley, eds)
pp. 113-117 (1994).
M. A. Benning, J. M. Kuo, F. M. Raushel and H.
M. Holden, "The Three-Dimensional
Structure of Phosphotriesterase: An Enzyme Capable of Detoxifying
Organophosphorus Nerve Agents" Biochemistry 33,
15001-15007 (1994).
Suk-bong
Hong
and Frank M. Raushel, "Synthesis and
Enzymatic Hydrolysis of a Light-Emitting Substrate for
Phosphotriesterase" Bioorganic & Medicinal
Chemistry Letters 4, 2705-2708 (1994).
B.
W. Miles, S. M. Mareya, L. E. Post, D. J. Post, S. H. Chang and F. M.
Raushel, "Differential Roles for
Three Conserved Histidine Residues Within the Large Subunit of
Carbamoyl Phosphate Synthetase" Biochemistry 32,
232-240 (1993).
E.
Kaliste-Korhonen, P. Ylitalo, O. Hanninen and F. M. Raushel
"Phosphotriesterase Decreases Paraoxon Toxicity in Mice" Toxicology
&
Applied Pharmacology 121, 275 (1993).
H.
M. Abu-Soud, A. C. Clark, W. A. Francisco, T. O. Baldwin and F. M.
Raushel, "Kinetic Destabilization of the Hydroperoxy
Flavin Intermediate by Site-Directed Modification of the Reactive
Thiol in Bacterial Luciferase" Journal of
Biological Chemistry 268, 7699-7706 (1993).
E.
J. Rodriguez, C. Debouck, I. Deckman, H. Abu-Soud, F. M. Raushel and
T. D. Meek, "Inhibitor Binding to
the Phe53Trp Mutant of HIV-1 Protease Promotes Conformational
Changes Detectable by Spectrofluorimetry" Biochemistry
32, 3557-3563 (1993).
R.
Rajapaksa, H. Abu-Soud, F. M. Raushel, B. G. Harris and P. F. Cook, "Pre-steady State
Kinetics Reveal a Slow Isomerization of the Enzyme-NAD Complex in
the NAD-Malic Enzyme Reaction" Biochemistry 32,
1928-1934 (1993).
L.
S. Mullins, C. N. Pace and F. M. Raushel, "Investigation of
Ribonuclease T1 Folding by Hydrogen Deuterium Amide Exchange-2D
NMR Spectroscopy" Biochemistry 32,
6152-6156 (1993).
G.
M. Omburo, L. S. Mullins and F. M. Raushel, "Structural
Characterization of the Divalent Cation Sites of Bacterial
Phosphotriesterase by 113Cd NMR Spectroscopy"
Biochemistry 32, 9148-9155 (1993).
W.
A. Francisco, H. Abu-Soud, T. O. Baldwin and F. M. Raushel, "Interaction of Aldehyde
Substrates and Inhibitors to Bacterial Luciferase" Journal
of Biological Chemistry 268, 24734-24741 (1993).
M. Y. Chae, G. E. Omburo, P. A. Lindahl and F.
M. Raushel, "Antiferromagnetic
Coupling in the Binuclear Metal Center of Mn-Substituted
Phosphotriesterase" Journal of American Chemical
Society 115, 12173-12174 (1993).
H.
M. Abu-Soud, L. S. Mullins, T. O. Baldwin and F. M. Raushel, "A Stopped-Flow Kinetic
Analysis of the Bacterial Luciferase Reaction" Biochemistry
31, 3807-3813 (1992).
B. L. Braxton, L. S. Mullins, F. M. Raushel
and G. D. Reinhart, "Quantifying the
Allosteric Properties of E.
coli Carbamyl Phosphate
Synthetase" Biochemistry 31, 2309-2316
(1992).
G.
A. Omburo, J. M. Kuo, L. S. Mullins and F. M. Raushel, "Characterization of the
Zinc Binding Site of Bacterial Phosphotriesterase" Journal
of Biological Chemistry 267, 13278-13283 (1992).
F.
M. Raushel, B. W. Miles, L. E. Post and D. J. Post, "Mutational Analysis of
Two Putative Domains Within the Large Subunit of Carbamoyl
Phosphate Synthetase from E.
coli" Bioorganic
& Medicinal Chemistry Letters 2, 319-322 (1992).
S.
R. Caldwell and F. M. Raushel, "Detoxification of Organophosphate
Pesticides Using an Immobilized Phosphotriesterase from Pseudomonas
diminuta" Biotechnology and Bioengineering 37,
103-109 (1991).
L.
S. Mullins, C. J. Lusty and F. M. Raushel, "Alterations in the
Energetics of Carbamoyl Phosphate Synthetase Reaction by
Site-Directed Modification of the Essential Sulfhydryl Group"
Journal of Biological Chemistry 266, 8236 (1991).
S.
R. Caldwell and F. M. Raushel, "Mechanism of Enzymatic Phosphotriester
Hydrolysis", in Chemical Aspects of Enzyme Biotechnology:
Fundamentals (T. O. Baldwin, F. M. Raushel, and A. I. Scott
eds.) Plenum Press 41-52 (1991).
F.
M. Raushel, H. M. Abu-Soud, L. S. Hester, W. Francisco and T. O.
Baldwin, "Kinetic Mechanism of Bacterial Luciferase" in Flavins and
Flavoproteins 1990 (Curti, Ronchi, and Zanetti, Eds.) Walter De Gruter
& Co. 273-276 (1991).
A.
L.
J. Chlumsky, L. H. Chen, C. Clark, H. Abu-Soud, M. M. Zeigler, F. M.
Raushel and T. O. Baldwin, "Random and Site Directed Mutagenesis of
Bacterial Luciferase" in Flavins and Flavoproteins 1990 (Curti,
Ronchi, and Zanetti, Eds.) Walter De Gruter & Co. 261-264 (1991).
S.
R. Caldwell, F. M. Raushel, P. M. Weiss and W. W. Cleland, "Primary and Secondary
Oxygen-18 Isotope Effects in the Chemical and Enzymatic Hydrolysis
of Phosphotriesters" Journal of American Chemical
Society 113, 730-732 (1991).
S.
R. Caldwell, J. R. Newcomb, K. A. Schlecht and F. M. Raushel, "Limits of Diffusion in
the Hydrolysis of Substrates by the Phosphotriesterase from Pseudomonas diminuta"
Biochemistry 30, 7438-7444 (1991).
S.
R. Caldwell, F. M. Raushel, P. M. Weiss and W. W. Cleland, "Transition State
Structures for the Enzymatic and Chemical Hydrolysis of
Phosphotriesters" Biochemistry 30,
7444-7450 (1991).
S.
R. Caldwell and F. M. Raushel "Detoxification of Organophosphate
Pesticides Using a Nylon Based Immobilized Phosphotriesterase from Pseudomonas
Diminuta" Applied Biochemistry & Biotechnology 31,
59-73 (1991).
S. G. Miran, S. H. Chang and F. M. Raushel, "The Role of the Four
Conserved Histidine Residues in the Amidotransferase Domain of
Carbamoyl Phosphate Synthetase" Biochemistry 30,
7901-7907 (1991).
J.
N. Blankenship, H. M. Abu-Soud, W. A. Francisco, F. M. Raushel, D. R.
Fischer and P. J. Stang, "Mechanism-Based
Inactivation of a Bacterial Phosphotriesterase by an Alkynyl
Phosphate Ester" Journal of American Chemical
Society 113, 8560-8561 (1991).
L.
S. Mullins, L. E. Zawadzke, C. T. Walsh and F. M. Raushel, "Kinetic Evidence for the
Formation of Alanyl-phosphate in the D-alanyl-alanine Synthetase
Reaction" Journal of Biological Chemistry 265,
8993-8998 (1990).
L. E. Post, D. J. Post and F. M. Raushel, "Dissection of the Functional Domains of E. coli
Carbamoyl Phosphate Synthetase by Site-Directed Mutagenesis"
Journal of Biological Chemistry 265, 7742-7747 (1990).
D. P. Dumas and F. M. Raushel, "Kinetic and Chemical
Evidence for an Essential Histidine Residue in the
Phosphotriesterase of Pseudomonas
diminuta" Journal
of Biological Chemistry 265, 21498-21503 (1990).
D.
P. Dumas, J. R. Wild and F. M. Raushel, "Expression of a Bacterial
Phosphotriesterase in the Fall Army Worm Confers Resistance to
Organophosphate Insecticides" Experientia 46, 729-731
(1990).
M.
McNutt, L. S. Mullins, F. M. Raushel and C. N. Pace "Contribution of
Histidine Residues to the Conformational Stability of Ribonuclease
T1 and Mutant Glu-58 to Ala" Biochemistry 29,
7572-7576 (1990).
D.
P. Dumas, J. R. Wild and F. M. Raushel, "Diisopropylfluorophosphate
Hydrolysis by an Organophosphate Anhydrase from Pseudomonas
diminuta" Biotechnology and Applied Biochemistry 11,
235-243 (1989).
W.
J. Donarski, D. P. Dumas, D. P. Heitmeyer, V. E. Lewis and F. M.
Raushel, "Structure-Activity
Relationships in the Hydrolysis of Substrates by the
Phosphotriesterase from Pseudomonas
diminuta" Biochemistry
28, 4650-4655 (1989).
D.
P. Dumas, S. R. Caldwell, J. R. Wild and F. M. Raushel, "Purification and
Properties of the Phosphotriesterase from Pseudomonas
diminuta" Journal
of Biological Chemistry 264, 19659-19665 (1989).
D.
P. Dumas, H. D. Durst, W. G. Landis, F. M. Raushel and J. R. Wild,
"Inactivation of Organophosphorus Nerve Agents by the
Phosphotriesterase from Pseudomonas diminuta" Archives of
Biochemistry & Biophysics 277, 155-159 (1989).
F.
M. Raushel and T. O. Baldwin, "Proposed Mechanism for the Bacterial
Luciferase Reaction Involving a Dioxirane Intermediate" Biochemical
& Biophysical Research Communications 164, 1137-1142
(1989).
V.
E. Lewis, W. J. Donarski, J. R. Wild and F. M. Raushel, "The Mechanism and
Stereochemical Course at Phosphorus of the Reaction Catalyzed by a
Bacterial Phosphotriesterase" Biochemistry 27,
1591-1597 (1988).
S.
C. Kim and F. M. Raushel, "The Interaction of Monofluorofumarate With
Adenylosuccinate Lyase" Journal of Enzyme Inhibition 2,
153-161 (1988).
A.
N. Singh, J. S. Newborn and F. M. Raushel, "Enzymatic Synthesis of
Uridine-5'-O-(2-thiodiphosphoglucose) and Related Sugar
Phosphorothioates" Bioorganic Chemistry 16, 206-214
(1988).
S.
C. Kim, A. N. Singh and F. M. Raushel, "The Mechanism of
Glycogen Synthetase as Determined by Deuterium Isotope Effects and
Positional Isotope Exchange Experiments" Journal of
Biological Chemistry 263, 10151-10154 (1988).
F.
M. Raushel and J. J. Villafranca, "Positional Isotope Exchange" Critical
Reviews in Biochemistry 23, 1-26 (1988).
S.
C. Kim, A. N. Singh and F. M. Raushel, "Analysis of the Galactosyl
Transferase Reaction by Positional Isotope Exchange and Secondary
Deuterium Isotope Effects" Archives of Biochemistry &
Biophysics 267, 54-59 (1988).
J.
D. Scholton, J. L. Hogg and F. M. Raushel, "Methyl Chymotrypsin
Catalyzed Hydrolyses of Specific Substrate Esters Indicate
Multiple Proton Catalysis is Possible with a Modified Charge Relay
Triad" Journal of American Chemical Society 110,
8246-8247 (1988).
S.
C. Kim and F. M. Raushel, "Mechanism-Based
Inactivation of Rabbit Muscle Phosphoglucomutase by
Nojirimycin-6-Phosphate" Biochemistry 27,
7328-7332 (1988).
L.S.Hester,
F.M.
Raushel, “Determination of the energetics of the UDP-glucose
pyrophosphorylase reaction by positional isotope exchange inhibition”
Biochemistry 26, 6465-6571 (1987).
L.S.Hester,
F.M.Raushel,
“Analysis of ping-pong reaction mechanisms by positional isotope
exchange. Application to galactose-1-phosphate uridyltransferase” J Biol Chem 262, 12092-12095 (1987).
L.M.Mallis,
F.M.
Raushel, D.H. Russell, “Differentiation of isotopically labeled
nucleotides using fast atom bombardment tandem mass spectrometry” Anal Chem 59, 980-984 (1987).
A.N.
Singh,
L.S. Hester, F.M. Raushel, “Examination of the mechanism of sucrose
synthetase by positional isotope exchange” J Biol Chem 262, 2554-2557 (1987).
J.R.
Matos,
F.M. Raushel, C.H. Wong, “S-adenosylmethionine: studies on chemical
and enzymatic synthesis” Biotechnol Appl Biochem 9,
39-52 (1987).
S.
C. Kim and F. M. Raushel, "The Determination of
Enzyme-Substrate Dissociation Rates by Dynamic Isotope Exchange
Enhancement Experiments" Journal of Biological
Chemistry 261, 8163-8166 (1986).
T. L. Chapman, T. B. Shull and F. M. Raushel,
"Stereochemical Probes of
the Argininosuccinate Synthetase Reaction" Biochemistry
25, 4739-4744 (1986).
S.
C. Kim and F. M. Raushel, "Isotopic Probes of the
Argininosuccinate Lyase Reaction" Biochemistry 25,
4744-4749 (1986).
L.
J. Garrard, Q. T. N. Bui, R. Nygaard and F. M. Raushel, "Acid-Base Catalysis in
the Argininosuccinate Lyase Reaction" Journal of
Biological Chemistry. 260, 5548-5553 (1985).
L.
W. Hilscher, C. Hanson, D. H. Russell and F. M. Raushel, "Measurement of
Positional Isotope Exchange Rates in Enzymes Catalyzed Reactions
by Fast Atom Bombardment Mass Spectrometry: Application to
Argininosuccinate Synthetase" Biochemistry 24,
5888-5893 (1985).
C. Ghose and F. M. Raushel, "Determination of the
Mechanism of the Argininosuccinate Synthetase Reaction by Static
and Dynamic Quench Experiments" Biochemistry 24,
5894-5898 (1985).
F.
M. Raushel and L. J. Garrard, "A Positional Isotope
Exchange Study of the Argininosuccinate Lyase Reaction"
Biochemistry 23, 1791-1795 (1984).
F.
M. Raushel, "Nitro Analogs of Substrates for Argininosuccinate
Synthetase and Argininosuccinate Lyase" Archives of Biochemistry
& Biophysics 232, 520-525 (1984).
F.
M. Raushel and R. Nygaard, "Kinetic Mechanism of Bovine Liver
Argininosuccinate Lyase" Archives of Biochemistry & Biophysics
221, 143-147 (1983).
F.
M. Raushel, P. M. Anderson and J. J. Villafranca, "An NMR Study of the
Topography of Binding Sites of E.
coli Carbamoyl Phosphate
Synthetase" Biochemistry 22, 1872-1876
(1983).
L.
J. Garrard, J. M. Mathis and F. M. Raushel, "Substrate-Induced
Inactivation of Argininosuccinate Lyase by Monofluorofumarate and
Difluorofumarate" Biochemistry 22,
3729-3735 (1983).
F.
M. Raushel and J. L. Seigle, "Kinetic Mechanism of Arginosuccinate
Synthetase" Archives of Biochemistry & Biophysics 225,
979-985 (1983).
R.
E. Viola, F. M. Raushel, A. R. Rendina and W. W. Cleland, "Substrate Synergism and
the Kinetic Mechanism of Yeast Hexokinase" Biochemistry
21, 1295-1302 (1982).
J.
J. Villafranca and F. M. Raushel, "NMR and EPR Investigations of
Bi-Metallo Enzymes" Advances in Inorganic Biochemistry 4,
289-319 (1982).
J.
J. Villafranca and F. M. Raushel, "The Monovalent Cation Site of
Pyruvate Kinase and Other Enzymes: NMR Investigations" Federation
Proceedings 41, 2961-2965 (1982).
B.
A. Cunningham, F. M. Raushel, J. J. Villafranca and S. J. Benkovic, "Distances Between
Structural Metal Ion, Substrates and the Allosteric Modifier of
Fructose Bisphosphatase" Biochemistry 20,
359-362 (1981).
J.
J. Villafranca, F. M. Raushel, R. P. Pillai, M. S. Balakrishnan, C.
DeBrosse and T. D. Meek, "31P NMR Studies of Phosphoryl
Transfer Enzymes" ACS Symposium Series, Phosphorus Chemistry 171,
131-134 (1981).
R.
P. Pillai, F. M. Raushel and J. J. Villafranca, "Stereochemistry of
Binding of Thiophosphate Analogs of ATP and ADP to Carbamate Kinase,
Glutamine Synthetase, and Carbamyl-Phosphate Synthetase" Archives
of Biochemistry & Biophysics 199, 7-15 (1980).
J.
J. Villafranca and F. M. Raushel, "Biophysical Application of NMR to
Phosphoryl Transfer Enzymes and Metal Nuclei of Metalloproteins" Annual
Review of Biophysics & Bioengineering 9, 363-392
(1980).
F.
M. Raushel and J. J. Villafranca, "31P NMR Application to the Positional Isotope
Exchange Reactions Catalyzed by E.
coli Carbamoyl Phosphate
Synthetase: Analysis of the Forward and Reverse Enzyme Reactions"
Biochemistry 19, 3170-3174 (1980).
F. M. Raushel and J. J. Villafranca, "A Direct NMR Method for
the Determination of Correlation Times in Enzyme Complexes
Involving Monovalent Cations and Paramagnetic Centers"
Journal of American Chemical Society 102, 6618-1689
(1980).
F.
M. Raushel and J. J. Villafranca "A Multinuclear NMR Study
of the Monovalent-Divalent Cation Sites of Pyruvate Kinase"
Biochemistry 19, 5481-5485 (1980).
F.
M. Raushel and J. J. Villafranca, "Determination of the
Rate-Limiting Steps of Escherichia
coli Carbamoyl Phosphate
Synthetase: Rapid Quench and Isotope Partitioning Experiments"
Biochemistry 18, 3424-3429 (1979).
F. M. Raushel, C. J. Rawding, P. M. Anderson
and J. J. Villafranca, "Paramagnetic Probes for
Carbamoyl Phosphate Synthetase: Metal Ion Binding Studies and
Preparation of Nitroxide Spin Labeled Derivatives" Biochemistry
18, 5562-5566 (1979).
J.
J. Villafranca and F. M. Raushel, "Metalloenzyme Catalysis" Advances
in Catalysis 28, 324-369 (1979).
F.
M. Raushel, P. M. Anderson and J. J. Villafranca, "Carbamoyl Phosphate
Synthetase of Escherichia
coli Uses the Same
Diastereomer of Adenosine-5'-2-thiotriphosphate at Both ATP Sites"
Journal of Biological Chemistry 253,
6627-6629 (1978).
F. M. Raushel, P. M. Anderson and J. J.
Villafranca, "Kinetic Mechanism of Escherichia coli
Carbamoyl Phosphate Synthetase" Biochemistry 17,
5587-5591 (1978).
F.
M.
Raushel and W. W. Cleland, "Bovine Liver
Fructokinase: Purification and Kinetic Properties" Biochemistry
16, 2169-2175 (1977).
F.
M. Raushel and W. W. Cleland, "Determination of the
Rate-Limiting Steps and Chemical Mechanism of Fructokinase by
Isotope Exchange, Isotope Partitioning, and pH Studies"
Biochemistry 16, 2169-2175 (1977).
F.
M.
Raushel and W. W. Cleland, "The Substrate and
Anomeic Specificity of Fructokinase," Journal of
Biological Chemistry 248, 8174-8177 (1973).
L.
E. Cook and F. M. Raushel, "Calculation of Retention Indices for
Benzene and Benzene Derivatives on the Basis of Molecular Structure" Journal
of Chromatography 65, 556-559 (1972).