Paul Lindahl Lab at Texas A&M University

Instrumentation

High-field Mössbauer

High-field Mossbauer

The SEE CO. high field Mössbauer spectrometer contains a cryostat with a variable field superconducting magnet, capable of taking data from 1.5K to 300K and 0 to 6T.

Low-field Mössbauer

Low-field Mossbauer

Two MS4WRC Spectrometers with a 4.5 to 300 K closed-cycle Helium-refrigerated system and a W106 temperature controller. Mössbauer spectroscopy allows us to observe all the iron present in our samples.

EPR

EPR

X-Band Bruker EMS Spectrometer with an Oxford ESR900 liquid Helium cryostat. EPR spectroscopy allows us to detect paramagnetic species present in our samples

UV-VIS

UV-VIS

Hitachi U-3310 UV-Vis Spectrometer with a Head-On Photomultiplier tube, for performing electron absorption experiments, namely determination of heme centers in isolated mitochondria and measurement of DNA, RNA or protein concentrations.

ICP-MS

icp-ms

Agilent Technologies 7700 Series ICP-MS with ASX-500 Series ICP-MS Autosampler, which allows us to determine the absolute concentrations of different metals in our samples.

Bioreactor

Bioreactor

3 Custom-designed 24 L bioreactors from ChemGlass. We need large batches of cell culture to obtain sufficient quantities of isolated organelles for analyses with Mössbauer and EPR spectroscopies.

FPLC

FPLC

The Fast Protein Liquid Chromatography instrument is an Akta FPLC system with variable configuration robotic fraction collector. It is housed in an anaerobic glovebox along with various columns for different modes, and has a custom designed splitter used to carry out parallel detection (ICP-MS) and fraction collection.

Biosafety cabinet

Biosafety Cabinet

NUAIRE LabGuard Class II, Type A2 Biosafety Cabinet. Used for manipulation of human cell cultures in a sterile environment.

Ultracentrifuge

UltraCentrifuge

Beckman Coulter Optima L-90K Ultracentrifuge with an SW 32 Ti rotor. Used for density gradient centrifugation of organelles to achieve greater purity of isolated organelles.

Glove box

Glove Box

MBraun LabMaster I30 Glove Box with Siemens Simatic Panel. The glove box has an Argon atmosphere, thus providing an anaerobic environment for isolation of organelles to preserve the oxidation states of iron-containing species in our samples.