TAMU logo
 
Welcome to
 
    The Begley Group

 

Texas A&M University, College Station, TX  
 

 

 

 

 

 

Selected Publications (last 5 yrs)

 
2017

[1] Wang, Y., Schnell, B., Baumann, S., Müller, R., and Begley, T. (2017) Biosynthesis of Branched Alkoxy groups: Iterative Methyl Group Alkylation by a Cobalamin-Dependent Radical SAM Enzyme. J. Am. Chem. Soc. 2017, 139 (5), pp 1742–1745.
[2] Rodrigues, M. J., Windeisen, V., Zhang, Y., Guédez, G., Weber, S., Strohmeier, M., Hanes, J. W., Royant, A., Evans, G., Sinning, I., Ealick, S. E., Begley, T. P., and Tews, I. (2017) Lysine Relay Mechanism Coordinates Intermediate Transfer in Vitamin B6 Biosynthesis. Nat. Chem. Biol. 13, 290–294
[3] Chakrabarty, Y., and Begley, T. P. (2017) Cannibalism Among the Flavins: A Novel C-N Bond Cleavage in Riboflavin Catabolism Mediated by a Flavin-Generated Superoxide Radical. Nat. Chem. Biol., under revision.

2016

[4] Zhang, X., Eser, B. E., Chanani, P. K., Begley, T. P., and Ealick, S. E. (2016) Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases, Biochemistry 55, 1826-1838.
[5] Xu, H., Chakrabarty, Y., Philmus, B., Mehta, A. P., Bhandari, D., Hohmann, H.-P., and Begley, T. P. (2016) Identification of the First Riboflavin Catabolic Gene Cluster Isolated from Microbacterium maritypicum G10, J. Biol. Chem. 291, 23506-23515.
[6] Nemeria, N. S., Shome, B., DeColli, A. A., Heflin, K., Begley, T. P., Meyers, C. F., and Jordan, F. (2016) Competence of Thiamin Diphosphate-Dependent Enzymes with 2'-Methoxythiamin Diphosphate Derived from Bacimethrin, a Naturally Occurring Thiamin Anti-vitamin, Biochemistry 55, 1135-1148.
[7] Jhulki, I., Chanani, P. K., Abdelwahed, S. H., and Begley, T. P. (2016) A Remarkable Oxidative Cascade That Replaces the Riboflavin C8 Methyl with an Amino Group During Roseoflavin Biosynthesis, J. Am. Chem. Soc. 138, 8324-8327.
[8] Fenwick, M. K., Philmus, B., Begley, T. P., and Ealick, S. E. (2016) Burkholderia glumae ToxA Is a Dual-Specificity Methyltransferase That Catalyzes the Last Two Steps of Toxoflavin Biosynthesis, Biochemistry 55, 2748-2759.
[9] Eser, B. E., Zhang, X., Chanani, P. K., Begley, T. P., and Ealick, S. E. (2016) From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis, J. Am. Chem. Soc. 138, 3639-3642.
[10] Bhandari, D. M., Fedoseyenko, D., and Begley, T. P. (2016) Tryptophan Lyase (NosL): A Cornucopia of 5'-Deoxyadenosyl Radical Mediated Transformations, J. Am. Chem. Soc. 138, 16184-16187.
[11] Adak, S., and Begley, T. P. (2016) Dibenzothiophene Catabolism Proceeds via a Flavin-N5-oxide Intermediate, J. Am. Chem. Soc. 138, 6424-6426.

2015

[12] Philmus, B., Shaffer, B. T., Kidarsa, T. A., Yan, Q., Raaijmakers, J. M., Begley, T. P., and Loper, J. E. (2015) Investigations into the Biosynthesis, Regulation, and Self-Resistance of Toxoflavin in Pseudomonas protegens Pf-5, ChemBioChem 16, 1782-1790.
[13] Philmus, B., Decamps, L., Berteau, O., and Begley, T. P. (2015) Biosynthetic Versatility and Coordinated Action of 5'-Deoxyadenosyl Radicals in Deazaflavin Biosynthesis, J. Am. Chem. Soc. 137, 5406-5413.
[14] Mehta, A. P., Abdelwahed, S. H., Mahanta, N., Fedoseyenko, D., Philmus, B., Cooper, L. E., Liu, Y., Jhulki, I., Ealick, S. E., and Begley, T. P. (2015) Radical S-Adenosylmethionine (SAM) Enzymes in Cofactor Biosynthesis: A Treasure Trove of Complex Organic Radical Rearrangement Reactions, J. Biol. Chem. 290, 3980-3986.
[15] Mehta, A. P., Abdelwahed, S. H., Fenwick, M. K., Hazra, A. B., Taga, M. E., Zhang, Y., Ealick, S. E., and Begley, T. P. (2015) Anaerobic 5-Hydroxybenzimidazole Formation from Aminoimidazole Ribotide: An Unanticipated Intersection of Thiamin and Vitamin B12 Biosynthesis, J. Am. Chem. Soc. 137, 10444-10447.
[16] Mehta, A. P., Abdelwahed, S. H., and Begley, T. P. (2015) Molybdopterin biosynthesis - Mechanistic Studies on a Novel MoaA Catalyzed Insertion of a Purine Carbon into the Ribose of GTP, Biochim. Biophys. Acta, Proteins Proteomics 1854, 1073-1077.
[17] Hazra, A. B., Han, A. W., Mehta, A. P., Mok, K. C., Osadchiy, V., Begley, T. P., and Taga, M. E. (2015) Anaerobic Biosynthesis of the Lower Ligand of Vitamin B12, Proc. Nat. Acad. Sci. U.S.A. 112, 10792-10797.
[18] Fenwick, M. K., Mehta, A. P., Zhang, Y., Abdelwahed, S. H., Begley, T. P., and Ealick, S. E. (2015) Non-canonical Active Site Architecture of the Radical SAM Thiamin Pyrimidine Synthase, Nat. Commun. 6, 6480.
[19] Bhandari, D. M., Xu, H., Nicolet, Y., Fontecilla-Camps, J. C., and Begley, T. P. (2015) Tryptophan Lyase (NosL): Mechanistic Insights from Substrate Analogues and Mutagenesis, Biochemistry 54, 4767-4769.

2014

[20] Mehta, A. P., Abdelwahed, S. H., Xu, H., and Begley, T. P. (2014) Molybdopterin Biosynthesis: Trapping of Intermediates for the MoaA-Catalyzed Reaction Using 2'-DeoxyGTP and 2'-ChloroGTP as Substrate Analogues, J. Am. Chem. Soc. 136, 10609-10614.
[21] McRose, D., Guo, J., Monier, A., Sudek, S., Wilken, S., Yan, S., Mock, T., Archibald, J. M., Begley, T. P., Reyes-Prieto, A., and Worden, A. Z. (2014) Alternatives to Vitamin B1 Uptake Revealed with Discovery of Riboswitches in Multiple Marine Eukaryotic Lineages, International Society for Microbial Ecology Journal 8, 2517-2529.
[22] Jurgenson, C. T., Ealick, S. E., and Begley, T. P. (2014) Biosynthesis of Thiamin Pyrophosphate, EcoSal Plus, 1-16.
[23] Cooper, L. E., O'Leary, S. E., and Begley, T. P. (2014) Biosynthesis of a Thiamin Antivitamin in Clostridium botulinum, Biochemistry 53, 2215-2217.

2013

[24] Soriano, E. V., Zhang, Y., Colabroy, K. L., Sanders, J. M., Settembre, E. C., Dorrestein, P. C., Begley, T. P., and Ealick, S. E. (2013) Active-site Models for Complexes of Quinolinate Synthase with Substrates and Intermediates, Acta Crystallogr., Sect D: Biol. Crystallogr. 69, 1685-1696.
[25] Sikowitz, M. D., Shome, B., Zhang, Y., Begley, T. P., and Ealick, S. E. (2013) Structure of a Clostridium botulinum C143S Thiaminase I/Thiamin Complex Reveals Active Site Architecture, Biochemistry 52, 7830-7839.
[26] Sikowitz, M. D., Cooper, L. E., Begley, T. P., Kaminski, P. A., and Ealick, S. E. (2013) Reversal of the Substrate Specificity of CMP N-Glycosidase to dCMP, Biochemistry 52, 4037-4047.
[27] Mehta, A. P., Hanes, J. W., Abdelwahed, S. H., Hilmey, D. G., Hanzelmann, P., and Begley, T. P. (2013) Catalysis of a New Ribose Carbon-Insertion Reaction by the Molybdenum Cofactor Biosynthetic Enzyme MoaA, Biochemistry 52, 1134-1136.
[28] Mehta, A. P., Abdelwahed, S. H., and Begley, T. P. (2013) Molybdopterin Biosynthesis: Trapping an Unusual Purine Ribose Adduct in the MoaA-Catalyzed Reaction, J. Am. Chem. Soc. 135, 10883-10885.
[29] Mahanta, N., Fedoseyenko, D., Dairi, T., and Begley, T. P. (2013) Menaquinone Biosynthesis: Formation of Aminofutalosine Requires a Unique Radical SAM Enzyme, J. Am. Chem. Soc. 135, 15318-15321.
[30] Hicks, K. A., O'Leary, S. E., Begley, T. P., and Ealick, S. E. (2013) Structural and Mechanistic Studies of HpxO, a Novel Flavin Adenine Dinucleotide-Dependent Urate Oxidase from Klebsiella pneumoniae, Biochemistry 52, 477-487.
[31] Gokulan, K., O'Leary, S. E., Russell, W. K., Russell, D. H., Lalgondar, M., Begley, T. P., Ioerger, T. R., and Sacchettini, J. C. (2013) Crystal Structure of Mycobacterium tuberculosis Polyketide Synthase 11 (PKS11) Reveals Intermediates in the Synthesis of Methyl-branched Alkylpyrones, J. Biol. Chem. 288, 16484-16494.
[32] Coquille, S., Roux, C., Mehta, A., Begley, T. P., Fitzpatrick, T. B., and Thore, S. (2013) Highresolution Crystal Structure of the Eukaryotic HMP-P Synthase (THIC) from Arabidopsis thaliana, Journal of Structural Biology 184, 438-444.
[33] Cooper, L. E., Fedoseyenko, D., Abdelwahed, S. H., Kim, S.-H., Dairi, T., and Begley, T. P. (2013) In Vitro Reconstitution of the Radical S-Adenosylmethionine Enzyme MqnC Involved in the Biosynthesis of Futalosine-Derived Menaquinone, Biochemistry 52, 4592-4594.

2012

[34] Philmus, B., Abdelwahed, S., Williams, H. J., Fenwick, M. K., Ealick, S. E., and Begley, T. P. (2012) Identification of the Product of Toxoflavin Lyase: Degradation via a Baeyer-Villiger Oxidation, J. Am. Chem. Soc. 134, 5326-5330.
[35] Lai, R.-Y., Huang, S., Fenwick, M. K., Hazra, A., Zhang, Y., Rajashankar, K., Philmus, B., Kinsland, C., Sanders, J. M., Ealick, S. E., and Begley, T. P. (2012) Thiamin Pyrimidine Biosynthesis in Candida albicans: A Remarkable Reaction Between Histidine and Pyridoxal Phosphate, J. Am. Chem. Soc. 134, 9157-9159.
[36] Huang, S., Mahanta, N., Begley, T. P., and Ealick, S. E. (2012) Pseudouridine Monophosphate Glycosidase: A New Glycosidase Mechanism, Biochemistry 51, 9245-9255.
[37] Greenwald, J. W., Greenwald, C. J., Philmus, B. J., Begley, T. P., and Gross, D. C. (2012) RNAseq Analysis Reveals that an ECF σ Factor, AcsS, Regulates Achromobactin Biosynthesis in Pseudomonas syringae pv. syringae B728a, PLoS One 7, e34804.
[38] Decamps, L., Philmus, B., Benjdia, A., White, R., Begley, T. P., and Berteau, O. (2012)
Biosynthesis of F0, Precursor of the F420 Cofactor Requires a Unique Two Radical-SAM Domain Enzyme and Tyrosine as Substrate, J. Am. Chem. Soc. 134, 18173-18176.
[39] Coats, D., Shelton-Dodge, K., Ou, K., Khun, V., Seab, S., Sok, K., Prou, C., Tortorelli, S., Moyer, T. P., Cooper, L. E., Begley, T. P., Enders, F., Fischer, P. R., and Topazian, M. (2012) Thiamine Deficiency in Cambodian Infants with and without Beriberi, Journal of Pediatrics (N. Y., NY, U. S.) 161, 843-847.
[40] Begley, T. P., Ealick, S. E., and McLafferty, F. W. (2012) Thiamin Biosynthesis: Still Yielding Fascinating Biological Chemistry, Biochem. Soc. Symp. 79, 115-124.

 

A Complete List of Publications
Research Interests| Prof. Tadhg Begley | Group Members |Publications | Photo Gallery | Useful Links