We are interested in high-performance analytical- and preparative-scale electrophoretic separations.
One part of our group designs, synthesizes and tests families of new, single-isomer, cyclodextrin-based chiral resolving agents for the analytical and preparative-scale electrophoretic separation of enantiomers in aqueous, hydro-organic and non-aqueous background electrolytes. The first family consists of per-substituted 6-O-sulfo cyclodextrins (α-, β- and γ-cyclodextrins) that carry identical groups (hydroxy, acetyl or methyl) at all of the 2- and 3-positions of the glucopyranose subunits of cyclodextrins. The second family consists of 6-O-sulfo cyclodextrins that carry non-identical groups at all of the 2- and 3- positions (e.g., 2-O-methyl-6-O-sulfo; 2-O-methyl-3-O-acetyl-6-O-sulfo; 2-O-methyl-3-O-sulfo; etc.). The latest family consists of mono-substituted and per-substituted multi-cationic cyclodextrins.
The second part of our group develops new methods, instruments and reagents for efficient analytical- and preparative-scale electrophoretic protein separations. Recently, we introduced pH biased isoelectric trapping to eliminate the major limitations of classical isoelectric trapping separations, both on the analytical-scale (proteomics studies) and the preparative-scale (reaching processing rates as high as 0.5 g/hour). We have developed new, hydrolytically and mechanically stable buffering membranes for use in these isoelectric trapping systems. We have synthesized and fully characterized new, single-component carrier ampholytes whose isoionic solutions have good buffering capacities and conductivities, for use as pH biasers in isoelectric trapping separations. We are working on new instrument designs that facilitate efficient Joule heat dissipation and offer both binary and simultaneous, multi-component protein separations.
Silvia Sanchez-Vindas and Gyula Vigh, "Non-aqueous capillary electrophoretic enantiomer separations using the tetrabutylammonium salt of heptakis(2,3-O-diacetyl-6-O-sulfo)-beta-cyclodextrin, a single-isomer sulfated cyclodextrin highly-soluble in organic solvents", J. of Chromatography A, 1068 (2005) 151-158.
Sanjiv Lalwani and Gyula Vigh, "A Family of High Buffering Capacity Diamino Sulfate Isoelectric Buffers for pH-Biased Isoelectric Trapping Separations", Electrophoresis, 26, (2005) 3-9.
Sanjiv Lalwani, Evan Shave and Gyula Vigh, "High Buffering Capacity, Hydrolytically-Stable, Low-pI Isoelectric Membranes for Isoelectric Trapping Separations", Electrophoresis, 25 (2004) 3323-3330.
Shulan Li and Gyula Vigh, "Use of the New, Single-Isomer, Single-Isomer Hexakis(2,3-diacetyl-6-O-sulfo)-α-cyclodextrin in Acidic Methanolic Background Electrolytes for Nonaqueous Capillary Electrophoretic Enantiomer Separations", J. Chromatogr. A. 1051 (2004) 95-102.
Shulan Li and Gyula Vigh, "Single-Isomer Sulfated α-Cyclodextrins for Capillary Electrophoresis. Part 3: Hexakis(2,3-di-O-methyl-6-O-sulfo)-α-cyclodextrin, Synthesis, Analytical Characterization and Initial Screening Tests", Electrophoresis, 25 (2004) 2657-2670.
Sanjiv Lalwani, Evan Shave, Helen C. Fleisher, Kingsley Nzeadibe, M. Brent Busby and Gyula Vigh, "Alkali-Stable High pI Isoelectric Membranes for Isoelectric Trapping Separations", Electrophoresis, 25 (2004) 2128 -2138.
Shulan Li and Gyula Vigh, "Single-Isomer Sulfated α-Cyclodextrins for Capillary Electrophoresis. Part 2: Hexakis(6-O-sulfo)-α-cyclodextrin, Synthesis, Analytical Characterization and Initial Screening Tests", Electrophoresis, 25 (2004) 1201-1210.
Evan Shave and Gyula Vigh, "Preparative Scale Isoelectric Trapping Separations in Methanol β Water Mixtures Using a Modified Gradiflow BF200IET Unit", J. Chromatogr. A. 1036 (2004) 3-6.
Evan Shave and Gyula Vigh, "Preparative Scale Recirculating, pH-Biased, Binary Isoelectric Trapping Separations", Electrophoresis 25 (2004) 381-387.