Leica Confocal

The MCF has recently acquired two new Leica TCS SP5 Confocal Laser Scanning Microscopes for fluorescence imaging of a wide range of materials and biological samples.

Leica TCS SP5 Confocal (room 341H)

This is an inverted DMI 6000 microscope which is equipped with a motorized xy stage and epifluorescence illumination. Filter cubes for blue and green excitation (I3, N2.1) are available for visual inspection and focusing of samples. The conventional scanner has one transmitted light detector and three reflected/fluorescence detectors. The excitation laser lines available are 458, 476, 488, 514, 543 and 633 nm and the microscope is equipped with 10x, 40x and 63x dry objectives and a 63x oil immersion objective.

Click for Confocal instructions

Leica TCS SP5 RS Confocal (room 341G)

This is an inverted DMI 6000 microscope which is equipped with a manual xy stage and epifluorescence illumination. Filter cubes for blue and green excitation (I3, N2.1) are available for visual inspection and focusing of samples. The resonant scanner has a scan speed of 8000 Hz (up to 25 frames per second at 512x512 pixels) and enables true confocal imaging and fast frame recording, a prerequisite for quantitative data recording of living samples. The system has one transmitted light detector and two reflected/fluorescence detectors. The excitation laser lines available are 458, 476, 488, 514, 543 and 633 nm and the microscope is equipped with 10x and 40x dry objectives and a 63x water immersion objective.

Click for Confocal instructions


For training on this instrument, please contact Dr. Amanda Young